Facilities

The general preparatory lab contains general equipment such as centrifuges, balances and water purification system.

Specialised equipment includes:

Nova Biomedial BioProfile 400

• Provides immediate analysis of key nutrients, metabolites, and gases in cell culture and fermentation media.
• Fully automated, multi-test systems designed for rapid, simultaneous analysis with results available in 3 minutes or less.
• Measured tests glucose, lactate, glutamine, glutamate, ammonium, pH, PO2, PCO2, sodium, potassium, acetate, phosphate, and glycerol.
  
  

Thermal-Induced Separation (TIPS) Apparatus

• Supercooled ethylene glycol bath attached to vacuum pump and liquid nitrogen

High Performance Liquid Chromatography System

• Equipped with amino acid exchange column

ELx 808 ELISA Reader

• Microplate reader to asses fluorescently-conjugated dye adherence to cells

Assorted centrifuges

• For 1.5 mL eppindorfs, 15 mL, and 50 mL centrifuge tubes
• Appartus to perform cytospin for cell imaging

Millipore GUAVA EasyCYTE Benchtop 8HT High Throughput Sampling Flow Cytometer

Key features:

• 2-laser setup: 488 nm, 640 nm for detecting up to 6 colors and 8 parameters.
• Automated sampling for 96 well plates / single sample loading for tubes
• Microcapillary flow cell for no sheath fluid
• Direct, absolute counts of cells per uL
• Guava InCYTE cytometry software

Ruskinn InvivO2 400 Hypoxia Workstation with Digital Microscope

Key features:

• Short interlock (pass-through) purge cycle time
• Single Plate Entry System (SPES)
• O2 stability from 0.0% (anoxia) to 20.9% (ambient) in 0.1% increments
• CO2 stability from 0.0% to 30.0% in 0.1% increments
• Digital microscope allows for the capture of images and movies for cells

Promega GloMax-Multi+ Detection System

Key features:

• Luminesence, Fluoresence and Absorbance for bioassays
• 6, 12, 48, 96, 382 well microplate reader
• Instinct Software with microplate builder on a color touch screen

BioFlo 110 bioreactor

Key features:

• Fermentation and cell culture system.
• Directs up to four culture vessels at once.
• A total of 32 control loops.
• Temperature control via water jacket.

Olympus BX51 digital image analysis fluorescence microscope

Key features:

• BX51 Olympus microscope
• UPLFL 10x objective
• UPLFL 20x objective
• Plan 50x oil objective
• UPLFL 100x oil objective
• BX-RFA Fluorescence illuminator
• U-MWIY2 wide band fluorescence cube (exciter BP 545/580 nm, DM 600, Barrier BA 610 nm)
• U-MNU2 narrow band fluorescence cube (exciter BP 360/370 nm, DM 500, Barrier BA 420 nm)
• U-MNB2 narrow band fluorescence cube (exciter 470/490 nm, DM500, Barrier BA 515 nm)
• DP50 Digital camera (5.8 million pixel equivalent)
• AnalySIS-Auto software for 3D image construction

Leica DM IL inverted phase microscope

Key features:

• Integrated Hoffman Modulation Contrast (IMC)
• Optimized Fluorescence with 3 position filter cube slider
• Field of View: 20 mm
• 6 Volt/35 Watt transmitted light illumination

This is a positive pressure clean room (HEPA-filtered air supply) equipped with three direct exhaust laminar flow cabinets.

Three (3x) HERAsafe Class II Biosafety Cabinets

Key features:

• Aerosol-tight & motor-driven front window.
• Filter rated at 99.999% for 0.3 µ particles.
• Windows made from non-reflecting laminated safety glass.
• A monitoring system that clearly alerts the user in the event of a blower or filter failure.
• A keypad lock to limit unauthorised access.

Varied Incubators (8x) designed for O2, CO2, static culture, and bioreactor outfits

Alongside a tissue culture room-specific microscope, centrifuge, and more.

Applied Biosystems StepOnePlus Real-Time PCR System (RT-PCR)

• Fast (40min) and Standard (2hr) runs
• PC-free or networked operation with intuitive software
• Supports high resolution melt (HRM) software
• 4 color, 96 well instrument
• VeriFlex block for PCR optimisation

With associated PCR shakers, thermocyclers, and more

Sygene BIOFLASH Gel Documentation System

• Darkroom (Epi white lighting factory fitted)
• High performance CCD camera with zoom lens
• For western blot / gene analysis

*If you do not have QuickTime Player, you can download it free .

 *If you do not have QuickTime Player, you can download it free.

Liquid Nitrogen Cell Cryobank

• For stable cell preservation

-80C Heracles freezer

• For medium, reagent, and powder storage

-20C freezer (3x)

4C refrigerator (3x)