Hands-on LC-MS for Metabolic Profiling
General Information
Introductory lectures in mass spectrometry and chromatography, study design and sample preparation, followed by preparation of biological samples for analysis on subsequent days.
DAY 1 | |
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Time | Topic |
9.00- 9.30 | Registration / Coffee |
9.30- 10.45 | Aims of course |
LC-MS for Metabolic Profiling | |
10.45-11.15 | Break |
11.15- 12.30 | Sample Prep - untargeted |
12.30-13.15 | Lunch |
13.15- 14.30 | Untargeted analysis sample preparation |
14.30-15.00 | Break |
15.00-16.30 | Targeted analysis sample preparation |
16.30-16.45 | Summary/Debrief session |
17.00-19.00 | Welcome Mixer |
Analysis of biofluids through global profiling and targeted analyses; an introductory session to liquid chromatography, followed by sessions spent on each of the newest QToF instrumentation and the newest TQ instrumentation. Instrument set up, method development and acquisition will be covered. As we have set a maximum of 3 attendees per instrument this allows for hands-on participation by all. Day 3 finishes with introduction to data analysis.
DAY 2 | |
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Time | Topic |
9.00 -9.30 | Coffee Break |
9.30- 10.45 | LC Session 1 |
10.45-11.15 | Break |
11.15- 12.30 | LC Session 1 |
12.30-13.15 | Lunch |
13.15 -14.30 | LC Session 1 |
14.30-15.00 | Break |
15.00-16.30 | LC Session 1 |
16.30-16.45 | Summary/Debrief Session |
18.00 | Course Dinner |
Analysis of biofluids through global profiling and targeted analyses; an introductory session to liquid chromatography, followed by sessions spent on each of the newest QToF instrumentation and the newest TQ instrumentation. Instrument set up, method development and acquisition will be covered. As we have set a maximum of 3 attendees per instrument this allows for hands-on participation by all. Day 3 finishes with introduction to data analysis.
DAY 3 | |
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Time | Topic |
9.00 -9.30 | Coffee Break |
9.30- 10.45 | MC Session 2 |
10.45-11.15 | Break |
11.15- 12.30 | MC Session 2 |
12.30-13.15 | Lunch |
13.15 -14.30 | Introduction to Statistics |
14.30-15.00 | Break |
15.00-16.30 | Further Statistics: OPLS |
Data analysis workshops where attendees will process the data acquired from the previous days, mixed with further statistics lectures, allowing for development of interpretation skills.
DAY 4 | |
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Time | Topic |
9.00-9.30 | Coffee Break |
9.30- 10.45 | Untargeted Data Analysis |
10.45-11.15 | Break |
11.15- 12.30 | Untargeted Data Analysis |
12.30-13.15 | Lunch |
13.15 -14.30 | Metabolite ID |
14.30-15.00 | Break |
15.00-16.30 | Targeted Data Analysis |
16.30-16.45 | Debrief |
Application lectures, tips, tricks and troubleshooting.
Day 5 | |
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Time | Topic |
9.00- 9.30 | Coffee Break |
9.30- 10.45 | Problem Solving Session |
10.45-11.15 | Break |
11.15- 12.30 | Problem Solving Session |
12.30-13.15 | Lunch |
13.15- 14.30 | Tips and Tricks for Metabolic Profiling |
14.30-15.00 | Break |
15.00-16.30 | Optional trip: National Centre |