LUMC
Growth media and agar compositions protocol.
Human macrophage differentiation protocol.
NKI
Salmonella Infection protocol
The mouse virulent strains S. typhimurium SL1344-Ds-Red was grown in Luria-Bertani (LB) media at 37°C in 5% CO2. HeLa or MCF7cells were seeded on 96 well plate at a density of 20/30 × 10EXP3 cells. Cells were cultured at 37°C in 5% CO2 for 48 h in DMEM media without penicillin/streptomycin. Bacteria were grown overnight at 37°C while shaking, subcultured at a dilution of 1:33 in fresh LB media, and incubated at 37°C while shaking for 3.5 h. Cells were infected with 50 bacteria/cell in DMEM media without antibiotics for 30 min at 37°C in 5% CO2. Infected cells were washed four to six times and incubated for 1 h in media containing 50 μg/ml gentamicin (Invitrogen). Infected cells were subsequently incubated for the indicated time points in media containing 10 μg/ml gentamicin. " (Marsman et al. , MBC, 2004)