Regeneration
Overview
We adopt root tip regeneration in Arabidopsis (Fig 1) as a model system to study plant tissue self-organization. The long-term goal is to infer universal effective theories about the emergence of order in multi-cellular systems.
The main approach is to combine standard molecular genetics methods with novel live imaging setups and quantitative assays, to dissect the molecular and physical mechanisms regulating tissue self-organization.
![Arabidopsis root regeneration. Longitudinal median optical sections of the same root, after its tip has been excised. One image / 24 hours](/media/migration/research-groups/icimages--tojpeg_1446562417323_x2.jpg)
Main questions
- Does the tissue reorganization depend on well-defined organizers (instructive) or merely on local cell-cell interactions (true self-organization)?
- How does organ regeneration differ from other examples of post-embryonic organogenesis?
- What are the genetic and physical mechanisms regulating and constraining root tip regeneration?
- How can such morphological dynamics be quantitated and mathematically analysed?
Light Sheet Fluorescence Microscopy
One of the tools used in this investigations is a unique setup based on light sheet microscopy, which allows us to acquire stacks of optical sections of growing and regenerating roots at cellular resolution (microns), with high temporal resolution (every 15 minutes) and for very long time (at least 7 days).
With this tool, we can observe in vivo the spatial and temporal dynamics of any fluorescent genetic reporter or molecular dye, during the entire duration of the regeneration process. The rich temporal series are used to test hypotheses on the fundamental mechanisms and to instruct ad hoc computational models.