In this section
@article{Gispert:2022:10.1073/pnas.2206563119,
author = {Gispert, Contamina I and Hindley, J and Pilkington, C and Shree, H and Barter, L and Ces, O and Elani, Y},
doi = {10.1073/pnas.2206563119},
journal = {Proceedings of the National Academy of Sciences of USA},
pages = {1--10},
title = {Stimuli-responsive vesicles as distributed artificial organelles for bacterial activation},
url = {http://dx.doi.org/10.1073/pnas.2206563119},
volume = {119},
year = {2022}
}
TY - JOUR
AB - Intercellular communication is a hallmark of living systems. As such, engineering artificial cells that possess this behavior has been at the heart of activities in bottom-up synthetic biology. Communication between artificial and living cells has potential to confer novel capabilities to living organisms that could be exploited in biomedicine and biotechnology. However, most current approaches rely on the exchange of chemical signals that cannot be externally controlled. Here, we report two types of remote-controlled vesicle-based artificial organelles that translate physical inputs into chemical messages that lead to bacterial activation. Upon light or temperature stimulation, artificial cell membranes are activated, releasing signaling molecules that induce protein expression in Escherichia coli. This distributed approach differs from established methods for engineering stimuli-responsive bacteria. Here, artificial cells (as opposed to bacterial cells themselves) are the design unit. Having stimuli-responsive elements compartmentalized in artificial cells has potential applications in therapeutics, tissue engineering, and bioremediation. It will underpin the design of hybrid living/nonliving systems where temporal control over population interactions can be exerted.
AU - Gispert,Contamina I
AU - Hindley,J
AU - Pilkington,C
AU - Shree,H
AU - Barter,L
AU - Ces,O
AU - Elani,Y
DO - 10.1073/pnas.2206563119
EP - 10
PY - 2022///
SN - 0027-8424
SP - 1
TI - Stimuli-responsive vesicles as distributed artificial organelles for bacterial activation
T2 - Proceedings of the National Academy of Sciences of USA
UR - http://dx.doi.org/10.1073/pnas.2206563119
UR - https://www.pnas.org/doi/full/10.1073/pnas.2206563119
UR - http://hdl.handle.net/10044/1/99709
VL - 119
ER -
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