In this section
@article{Cavallo:2021:10.1021/acssensors.0c02605,
author = {Cavallo, FR and Mirza, KB and de, Mateo S and Nikolic, K and Rodriguez-Manzano, J and Toumazou, C},
doi = {10.1021/acssensors.0c02605},
journal = {ACS Sensors},
pages = {709--715},
title = {Aptasensor for quantification of leptin through PCR amplification of short DNA-aptamers.},
url = {http://dx.doi.org/10.1021/acssensors.0c02605},
volume = {6},
year = {2021}
}
TY - JOUR
AB - Protein quantification is traditionally performed through enzyme-linked immunosorbent assay (ELISA), which involves long preparation times. To overcome this, new approaches use aptamers as an alternative to antibodies. In this paper, we present a new approach to quantify proteins with short DNA aptamers through polymerase chain reaction (PCR) resulting in shorter protocol times with comparatively improved limits of detection. The proposed method includes a novel way to quantify both the target protein and the corresponding short DNA-aptamers simultaneously, which also allows us to fully characterize the performance of aptasensors. Human leptin is used as a target protein to validate this technique, because it is considered an important biomarker for obesity-related studies. In our experiments, we achieved the lowest limit of detection of 100 pg/mL within less than 2 h, a limit affected by the dissociation constant of the leptin aptamer, which could be improved by selecting a more specific aptamer. Because of the simple and inexpensive approach, this technique can be employed for Lab-On-Chip implementations and for rapid "on-site" quantification of proteins.
AU - Cavallo,FR
AU - Mirza,KB
AU - de,Mateo S
AU - Nikolic,K
AU - Rodriguez-Manzano,J
AU - Toumazou,C
DO - 10.1021/acssensors.0c02605
EP - 715
PY - 2021///
SN - 2379-3694
SP - 709
TI - Aptasensor for quantification of leptin through PCR amplification of short DNA-aptamers.
T2 - ACS Sensors
UR - http://dx.doi.org/10.1021/acssensors.0c02605
UR - https://www.ncbi.nlm.nih.gov/pubmed/33650854
UR - https://pubs.acs.org/doi/10.1021/acssensors.0c02605
VL - 6
ER -
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