@article{Kalofonou:2013:10.1016/j.snb.2012.12.054,
author = {Kalofonou, M and Toumazou, C},
doi = {10.1016/j.snb.2012.12.054},
journal = {SENSORS AND ACTUATORS B-CHEMICAL},
pages = {572--580},
title = {Semiconductor technology for early detection of DNA methylation for cancer: From concept to practice},
url = {http://dx.doi.org/10.1016/j.snb.2012.12.054},
volume = {178},
year = {2013}
}

Download

TY  - JOUR
AB  - The electrical detection of DNA methylation based biomarkers using semiconductor technology shows great promise for early cancer screening. Presented is the very first proof-of-concept example of using CMOS-based technology for real-time DNA methylation detection using Ion-Sensitive Field-Effect Transistors (ISFETs). An electrochemical label-free approach was applied in two gene assays, each one of which incorporated the sequences of DAPK1 and CDKN2A/p16-INK4 (p16) gene promoters at a both methylated and unmethylated state, performing isothermal methylation-specific amplification and detection both in-tube and on-chip (real-time). Good discrimination was shown between the two states, achieving a very good average pH signal change for the methylated state of 1.91 for DAPK1 assay and of 1.58 for p16 assay in the tube test. The real-time on-chip test showed similarly good real-time differential signal change in favour of methylated DNA, reaching 37 mV for DAPK1 assay and 23 mV for p16 assay, validating the results from the proof-of-concept test of pH-LAMP in-tube while confirming the sensitivity of real-time methylation-specific pH-LAMP on-chip.
AU  - Kalofonou,M
AU  - Toumazou,C
DO  - 10.1016/j.snb.2012.12.054
EP  - 580
PY  - 2013///
SN  - 0925-4005
SP  - 572
TI  - Semiconductor technology for early detection of DNA methylation for cancer: From concept to practice
T2  - SENSORS AND ACTUATORS B-CHEMICAL
UR  - http://dx.doi.org/10.1016/j.snb.2012.12.054
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000315751100075&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
VL  - 178
ER  - 

Download