@article{Toumazou:2013:10.1038/nmeth.2520,
author = {Toumazou, C and Shepherd, LM and Reed, S and Chen, GI and Patel, A and Garner, DM and Wang, CA and Ou, CP and Amin-Desai, K and Athanasiou, P and Bai, H and Brizido, IMQ and Caldwell, B and Coomber-Alford, D and Georgiou, P and Jordan, KS and Joyce, JC and La, Mura M and Morley, D and Sathyavruthan, S and Temelso, S and Temelso, RE and Zhang, L},
doi = {10.1038/nmeth.2520},
journal = {Nature Methods},
title = {Simultaneous DNA amplification and detection using a pH-sensing semiconductor system},
url = {http://dx.doi.org/10.1038/nmeth.2520},
volume = {10},
year = {2013}
}

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TY  - JOUR
AB  - We developed an integrated chip for real-time amplification and detection of nucleic acid using pH-sensing complementary metal-oxide semiconductor (CMOS) technology. Here we show an amplification-coupled detection method for directly measuring released hydrogen ions during nucleotide incorporation rather than relying on indirect measurements such as fluorescent dyes. This is a label-free, non-optical, real-time method for detecting and quantifying target sequences by monitoring pH signatures of native amplification chemistries. The chip has ion-sensitive field effect transistor (ISFET) sensors, temperature sensors, resistive heating, signal processing and control circuitry all integrated to create a full system-on-chip platform. We evaluated the platform using two amplification strategies: PCR and isothermal amplification. Using this platform, we genotyped and discriminated unique single-nucleotide polymorphism (SNP) variants of the cytochrome P450 family from crude human saliva. We anticipate this semiconductor technology will enable the creation of devices for cost-effective, portable and scalable real-time nucleic acid analysis.
AU  - Toumazou,C
AU  - Shepherd,LM
AU  - Reed,S
AU  - Chen,GI
AU  - Patel,A
AU  - Garner,DM
AU  - Wang,CA
AU  - Ou,CP
AU  - Amin-Desai,K
AU  - Athanasiou,P
AU  - Bai,H
AU  - Brizido,IMQ
AU  - Caldwell,B
AU  - Coomber-Alford,D
AU  - Georgiou,P
AU  - Jordan,KS
AU  - Joyce,JC
AU  - La,Mura M
AU  - Morley,D
AU  - Sathyavruthan,S
AU  - Temelso,S
AU  - Temelso,RE
AU  - Zhang,L
DO  - 10.1038/nmeth.2520
PY  - 2013///
SN  - 1548-7105
TI  - Simultaneous DNA amplification and detection using a pH-sensing semiconductor system
T2  - Nature Methods
UR  - http://dx.doi.org/10.1038/nmeth.2520
VL  - 10
ER  - 

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