Microscopy and Imaging

Specifications

• Microscope
  • DMi8CEL inverted 
  • Scanning stage
  • Universal insert
  • Microplate insert
  • LED illumination with DAPI, FITC and RHOD filters

• Lasers
  • 405 UV laser
  • Super continuum white light laser (470-670nm)

• Objectives

• Detectors
  • 1x PMT
  • 2x HyD

• Environmental control
  • Temperature

• Extras
  • Lightning

Specifications

• Microscope
  • DMi 6000CS inverted 
  • Scanning stage
  • Universal insert
  • Microplate insert
  • SuperZ galvo
  • Mercury lamp illumination with DAPI, FITC and RHOD filters

• Lasers
  • Argon: 458nm, 476nm, 488 nm, 496nm and 514nm
  • DPSS: 561nm
  • HeNe: 633nm

• Objectives
  • PL APO PH 10x/0.40 dry (WD 2.2mm)
  • PL APO 20x/0.70 IMM (WD 0.26mm water - 0.17mm oil)
  • PL APO PH 40x/1.25 oil (0.1mm)
  • PL APO 63x/1.3 Glycerol (0.28mm)

• Detectors
  • 3x PMT

• Environmental control
  • Temperature

Light microscopes	Make/Model	Features
	Leica DM IL​	Objectives: ​4x, 10x, 20x, 40x Inverted with Phase contrast Attached to Leica DFC295 Camera
​	Leica DM IL​	Objectives: ​4x, 10x, 20x, 40x  Inverted with Phase contrast  ​Attached to Leica DFC290 Camera
​	​Motic AE 2000	​Objectives: ​4x, 10x, 20x, 40x Inverted with Phase contrast Attached to Moticam 2.0MP Camera
	​Leica MZ6 dissecting stereomicroscope	​Widefield adjustable eyepieces Schott LED gooseneck lighting
	​Brunel dissecting microscope	​Widefield adjustable eyepieces Schott LED gooseneck lighting
	Zeiss stereomicroscope Stemi DV4	​4x zoom and 100% focus View your samples with precise focus through the full zoom range: From 8x to an impressive 32x magnification Integrated fiber-optic cold light illumination for a fully illuminated field of view
​	Motic SMZ-171 Series Stereo Zoom microscope​	Integrated LED illumination for a fully illuminated field of view. Attached to a tablet camera VisiCam TC 10 to allow image acquisition.

Fluorescence microscopes

	Make/Model	Features
​	Zeiss Axioplan epifluorescenc​e microscope	​Upright Epifluorescence and transmitted light (bright field and phase contrast) Objectives: 4x, 10x, 20x (Phase), 40x (oil), 100x (oil) Fluorescence channels : DAPI, FITC, TRITC Fitted with Leica DFC 310 FX camera
​​	Brunel SP105F​ with pE300 LED​	Inverted fluorescence microscope; Transmitted illumination - 6v 30watt (bright field and phase contrast available) Objectives: 10x,​ 20x, 40x Fluorescence channels: UV1 - DAPI, B1 – FITC, G1 – TexasRED Fitted with Canon EOS 1200D camera	​

Specifications

• Microscope
  • DMi8 CS inverted 
  • XY scanning stage
  • Z Galvo
  • Universal insert
  • Microplate insert
  • LED illumination with DAPI, 470, RHOD and TXR filters

• Lasers
  • 405nm
  • 442nm
  • Argon: 458nm, 476nm, 488nm, 496nm and 514nm
  • DPSS: 561nm
  • HeNe: 633nm

• Objectives

• Detectors
  • 2x PMT
  • 3x HyD GaAsP 

• Extras
  • Lightning
  • FRAP
  • FRET
  • 3D Visualiser
  • Live Data Mode

Description

Confocal microscope without environmental control

Specifications

• Microscope
  • DMi8 CS inverted 
  • XY scanning stage
  • Z Galvo
  • Universal insert
  • Microplate insert
  • LED illumination with DAPI, 470, RHOD and TXR filters

• Lasers
  • 405nm
  • 442nm
  • Argon: 458nm, 476nm, 488nm, 496nm and 514nm
  • DPSS: 561nm
  • HeNe: 633nm

• Objectives

• Detectors
  • 2x PMT
  • 3x HyD GaAsP 

• Extras
  • Lightning
  • FRAP
  • FRET
  • 3D Visualiser
  • Live Data Mode

Specs coming soon

Imaging:

Spatial Resolution : 0.7 μm

Minimum Achievable Voxel : 70 nm

Resolution at a Distance (RaaD) at 50 mm working distance : 1 μm

X-ray source:

Type: Seal-transmission

Tube voltage range: 30 - 160 kV

Maximum output: 10 W

Detector system: ZEISS X-ray microscopes feature a detector turret with multiple objectives at different magnifications. Each objective features optimized scintillators that deliver the highest absorption contrast details. 

Objectives: 0.4X, 4X and 20X

PC available for image analysis using Imaris and LASX

Specifications

• Imaris
• LASX

Links

• https://imaris.oxinst.com/tutorials
• https://www.youtube.com/channel/UCWMIU-_EfmXtY6AmtWReBeQ

Access protocol

Room safety induction and training required before access. Please request training in PPMS: https://ppms.eu/imperial/areq/?pf=5

-- You are responsible for your data. --

Please move your data from the acquisition computers.

• They really aren't safe places to keep anything
• Please either move the data (rather than copy) or delete the local copies after you have them safely elsewhere
• You may keep a copy of your data on a microscope computer for up to one week while you are making sure you have it backed up to your own lab based storage location.
• We may delete any files without warning if space is required for experiments
• (Caveat for small files, we understand sometime it is useful to store a reference file (eg to reload settings) so it is fine to keep permanently a small volume of data in a clearly labeled folder)
• Any files on any computer should be in a folder with your full name or netid on the data drive (please don't use the desktop or "my documents" as it buries the files in places less easy to maintain.