In this section

Publications

Selected publications

Feuerstein D, Manning A, Hashemi P, et al., Dynamic metabolic response to multiple spreading depolarizations in patients with acute brain injury: an online microdialysis study, Journal of Cerebral Blood Flow and Metabolism Vol:30, ISSN:0271-678X, Pages:1343-1355.
Feuerstein D, Parker KH, Boutelle MG, 2009, Practical Methods for Noise Removal: Applications to Spikes, Nonstationary Quasi-Periodic Noise, and Baseline Drift, Analytical Chemistry, Vol:81, ISSN:0003-2700, Pages:4987-4994.
Hashemi P, Bhatia R, Nakamura H et al.,2009, Persisting depletion of brain glucose following cortical spreading depression, despite apparent hyperaemia: evidence for risk of an adverse effect of Leao's spreading depression, Journal of Cerebral Blood Flow and Metabolism, Vol:29, ISSN:0271-678X, Pages:166-175. Author web link.
Fabricius M, Fuhr S, Willumsen L etal., 2008, Association of seizures with cortical spreading depression and peri-infarct depolarisations in the acutely injured human brain, Clinical Neurophysiology, Vol:119, ISSN:1388-2457, Pages:1973-1984.
Caesar K, Hashemi P, Douhou A, et al., 008, Glutamate receptor-dependent increments in lactate, glucose and oxygen metabolism evoked in rat cerebellum in vivo, Journal of Physiology - London, Vol:586, ISSN:0022-3751, Pages:1337-1349.

Citation

BibTex format

@article{Corcoles:2011:10.11113/jt.v54.800,
author = {Corcoles, EP and Deeb, S and Hanna, G and Boutelle, MG and Darzi, A},
doi = {10.11113/jt.v54.800},
journal = {Jurnal Teknologi (Sciences and Engineering)},
pages = {47--59},
title = {Adaptation of on-line rapid sampling microdialysis to monitor human intestinal ischaemia},
url = {http://dx.doi.org/10.11113/jt.v54.800},
volume = {54},
year = {2011}
}

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RIS format (EndNote, RefMan)

TY - JOUR
AB - Intestinal ischaemia or poor perfusion of the gastrointestinal tract is a major cause of post-operative mortality in abdominal surgery. Currently diagnosis of ischaemia relies only on clinical symptoms. Thus, monitoring bowel metabolism as an early marker of intestinal ischaemia is necessary. Human bowel microdialysis has been used in the past to study the metabolism of ichaemia collecting dialysate samples from the peritoneal cavity every 60 minutes. These sampling times carry a long delay for the detection of the typically rapid ischaemic event. We have previously developed a successful biosensor system to monitor neurochemicals in the human brain during surgery and in the intensive care unit The method consists of a flow injection analysis (FIA) system coupled to an enzyme based amperometric detector. The rapid sampling microdialysis monitoring system analysed electrochemically the dialysate glucose and lactate at high time resolution (typically 30 second sampling). Adaptation of the analytical assay system for on-line microdialysis monitoring of human bowel was performed and validated for in vivo procedures. Optimum membranes loading ratios were found to be 1:0.5 GOx:HRP and 2:0.5 LOx:HRP. The ischemic range was found to be 15μM-400μM, 40μM-6mM for glucose and lactate, respectively. The calibration method for these monitorings was concluded with a range from 250 μM to 6 mM. © Universiti Teknologi Malaysia.
AU - Corcoles,EP
AU - Deeb,S
AU - Hanna,G
AU - Boutelle,MG
AU - Darzi,A
DO - 10.11113/jt.v54.800
EP - 59
PY - 2011///
SN - 0127-9696
SP - 47
TI - Adaptation of on-line rapid sampling microdialysis to monitor human intestinal ischaemia
T2 - Jurnal Teknologi (Sciences and Engineering)
UR - http://dx.doi.org/10.11113/jt.v54.800
VL - 54
ER -

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Professor Martyn Boutelle
Department of Bioengineering
Room 3.14, Royal School of Mines Building
Imperial College London
London, SW7 2AZ

Tel: +44 (0)20 7594 5138
Email: m.boutelle@imperial.ac.uk

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