In this section
@article{Zaveri:2021:10.1016/j.bpj.2020.11.008,
author = {Zaveri, A and Bose, A and Sharma, S and Rajendran, A and Biswas, P and Shenoy, AR and Visweswariah, SS},
doi = {10.1016/j.bpj.2020.11.008},
journal = {Biophysical Journal},
pages = {1231--1246},
title = {Mycobacterial STAND adenylyl cyclases: the HTH domain binds DNA to form biocrystallized nucleoids},
url = {http://dx.doi.org/10.1016/j.bpj.2020.11.008},
volume = {120},
year = {2021}
}
TY - JOUR
AB - Mycobacteria harbor a unique class of adenylyl cyclases with a complex domain organization consisting of an N-terminal putative adenylyl cyclase domain fused to a nucleotide-binding adaptor shared by apoptotic protease-activating factor-1, plant resistance proteins, and CED-4 (NB-ARC) domain, a tetratricopeptide repeat (TPR) domain, and a C-terminal helix-turn-helix (HTH) domain. The products of the rv0891c-rv0890c genes represent a split gene pair, where Rv0891c has sequence similarity to adenylyl cyclases, and Rv0890c harbors the NB-ARC-TPR-HTH domains. Rv0891c had very low adenylyl cyclase activity so it could represent a pseudoenzyme. By analyzing the genomic locus, we could express and purify Rv0890c and find that the NB-ARC domain binds ATP and ADP, but does not hydrolyze these nucleotides. Using systematic evolution of ligands by exponential enrichment (SELEX), we identified DNA sequences that bound to the HTH domain of Rv0890c. Uniquely, the HTH domain could also bind RNA. Atomic force microscopy revealed that binding of Rv0890c to DNA was sequence independent, and binding of adenine nucleotides to the protein induced the formation of higher order structures that may represent biocrystalline nucleoids. This represents the first characterization of this group of proteins and their unusual biochemical properties warrant further studies into their physiological roles in future.
AU - Zaveri,A
AU - Bose,A
AU - Sharma,S
AU - Rajendran,A
AU - Biswas,P
AU - Shenoy,AR
AU - Visweswariah,SS
DO - 10.1016/j.bpj.2020.11.008
EP - 1246
PY - 2021///
SN - 0006-3495
SP - 1231
TI - Mycobacterial STAND adenylyl cyclases: the HTH domain binds DNA to form biocrystallized nucleoids
T2 - Biophysical Journal
UR - http://dx.doi.org/10.1016/j.bpj.2020.11.008
UR - https://www.ncbi.nlm.nih.gov/pubmed/33217386
UR - https://www.sciencedirect.com/science/article/pii/S0006349520308961
UR - http://hdl.handle.net/10044/1/85560
VL - 120
ER -
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