In this section
@article{Solntceva:2021:10.3389/fcimb.2020.621452,
author = {Solntceva, V and Kostrzewa, M and Larrouy-Maumus, G},
doi = {10.3389/fcimb.2020.621452},
journal = {Frontiers in Cellular and Infection Microbiology},
title = {Detection of species-specific lipids by routine MALDI TOF mass spectrometry to unlock the challenges of microbial identification and antimicrobial susceptibility testing},
url = {http://dx.doi.org/10.3389/fcimb.2020.621452},
volume = {10},
year = {2021}
}
TY - JOUR
AB - MALDI-TOF mass spectrometry has revolutionized clinical microbiology diagnostics by delivering accurate, fast, and reliable identification of microorganisms. It is conventionally based on the detection of intracellular molecules, mainly ribosomal proteins, for identification at the species-level and/or genus-level. Nevertheless, for some microorganisms (e.g., for mycobacteria) extensive protocols are necessary in order to extract intracellular proteins, and in some cases a protein-based approach cannot provide sufficient evidence to accurately identify the microorganisms within the same genus (e.g., Shigella sp. vs E. coli and the species of the M. tuberculosis complex). Consequently lipids, along with proteins are also molecules of interest. Lipids are ubiquitous, but their structural diversity delivers complementary information to the conventional protein-based clinical microbiology matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) based approaches currently used. Lipid modifications, such as the ones found on lipid A related to polymyxin resistance in Gram-negative pathogens (e.g., phosphoethanolamine and aminoarabinose), not only play a role in the detection of microorganisms by routine MALDI-TOF mass spectrometry but can also be used as a read-out of drug susceptibility. In this review, we will demonstrate that in combination with proteins, lipids are a game-changer in both the rapid detection of pathogens and the determination of their drug susceptibility using routine MALDI-TOF mass spectrometry systems.
AU - Solntceva,V
AU - Kostrzewa,M
AU - Larrouy-Maumus,G
DO - 10.3389/fcimb.2020.621452
PY - 2021///
SN - 2235-2988
TI - Detection of species-specific lipids by routine MALDI TOF mass spectrometry to unlock the challenges of microbial identification and antimicrobial susceptibility testing
T2 - Frontiers in Cellular and Infection Microbiology
UR - http://dx.doi.org/10.3389/fcimb.2020.621452
UR - http://hdl.handle.net/10044/1/86333
VL - 10
ER -
CBRB
Imperial College London
Flowers Building
Exhibition Road
London SW7 2AZ
