@article{Costa:2021:10.1111/mmi.14670,
author = {Costa, TRD and Harb, L and Khara, P and Zeng, L and Hu, B and Christie, PJ},
doi = {10.1111/mmi.14670},
journal = {Molecular Microbiology},
pages = {436--452},
title = {Type IV secretion systems: Advances in structure, function, and activation},
url = {http://dx.doi.org/10.1111/mmi.14670},
volume = {115},
year = {2021}
}
TY - JOUR
AB - Bacterial type IV secretion systems (T4SSs) are a functionally diverse translocation superfamily. They consist mainly of two large subfamilies: (i) conjugation systems that mediate interbacterial DNA transfer and (ii) effector translocators that deliver effector macromolecules into prokaryotic or eukaryotic cells. A few other T4SSs export DNA or proteins to the milieu, or import exogenous DNA. The T4SSs are defined by 6 or 12 conserved "core" subunits that respectively elaborate "minimized" systems in Gram-positive or -negative bacteria. However, many "expanded" T4SSs are built from "core" subunits plus numerous others that are system-specific, which presumptively broadens functional capabilities. Recently, there has been exciting progress in defining T4SS assembly pathways and architectures using a combination of fluorescence and cryoelectron microscopy. This review will highlight advances in our knowledge of structure-function relationships for model Gram-negative bacterial T4SSs, including "minimized" systems resembling the Agrobacterium tumefaciens VirB/VirD4 T4SS and "expanded" systems represented by the Helicobacter pylori Cag, Legionella pneumophila Dot/Icm, and F plasmid-encoded Tra T4SSs. Detailed studies of these model systems are generating new insights, some at atomic resolution, to long-standing questions concerning mechanisms of substrate recruitment, T4SS channel architecture, conjugative pilus assembly, and machine adaptations contributing to T4SS functional versatility.
AU - Costa,TRD
AU - Harb,L
AU - Khara,P
AU - Zeng,L
AU - Hu,B
AU - Christie,PJ
DO - 10.1111/mmi.14670
EP - 452
PY - 2021///
SN - 0950-382X
SP - 436
TI - Type IV secretion systems: Advances in structure, function, and activation
T2 - Molecular Microbiology
UR - http://dx.doi.org/10.1111/mmi.14670
UR - https://www.ncbi.nlm.nih.gov/pubmed/33326642
UR - https://onlinelibrary.wiley.com/doi/10.1111/mmi.14670
UR - http://hdl.handle.net/10044/1/110994
VL - 115
ER -