BibTex format
@article{Kamal:2021:10.1164/rccm.202103-0660OC,
author = {Kamal, F and Kumar, S and Edwards, MR and Veselkov, K and Belluomo, I and Kebadze, T and Romano, A and Trujillo-Torralbo, M-B and Shahridan, Faiez T and Walton, R and Ritchie, AI and Wiseman, DJ and Laponogov, I and Donaldson, G and Wedzicha, JA and Johnston, SL and Singanayagam, A and Hanna, GB},
doi = {10.1164/rccm.202103-0660OC},
journal = {American Journal of Respiratory and Critical Care Medicine},
pages = {1075--1085},
title = {Virus-induced volatile organic compounds are detectable in exhaled breath during pulmonary infection.},
url = {http://dx.doi.org/10.1164/rccm.202103-0660OC},
volume = {204},
year = {2021}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a condition punctuated by acute exacerbations commonly triggered by viral and/or bacterial infection. Early identification of exacerbation trigger is important to guide appropriate therapy but currently available tests are slow and imprecise. Volatile organic compounds (VOCs) can be detected in exhaled breath and have the potential to be rapid tissue-specific biomarkers of infection aetiology. METHODS: We used serial sampling within in vitro and in vivo studies to elucidate the dynamic changes that occur in VOC production during acute respiratory viral infection. Highly sensitive gas-chromatography mass spectrometry (GC-MS) techniques were used to measure VOC production from infected airway epithelial cell cultures and in exhaled breath samples of healthy subjects experimentally challenged with rhinovirus A16 and COPD subjects with naturally-occurring exacerbations. RESULTS: We identified a novel VOC signature comprising of decane and other related long chain alkane compounds that is induced during rhinovirus infection of cultured airway epithelial cells and is also increased in the exhaled breath of healthy subjects experimentally challenged with rhinovirus and of COPD patients during naturally-occurring viral exacerbations. These compounds correlated with magnitude of anti-viral immune responses, virus burden and exacerbation severity but were not induced by bacterial infection, suggesting they represent a specific virus-inducible signature. CONCLUSION: Our study highlights the potential for measurement of exhaled breath VOCs as rapid, non-invasive biomarkers of viral infection. Further studies are needed to determine whether measurement of these signatures could be used to guide more targeted therapy with antibiotic/antiviral agents for COPD exacerbations.
AU - Kamal,F
AU - Kumar,S
AU - Edwards,MR
AU - Veselkov,K
AU - Belluomo,I
AU - Kebadze,T
AU - Romano,A
AU - Trujillo-Torralbo,M-B
AU - Shahridan,Faiez T
AU - Walton,R
AU - Ritchie,AI
AU - Wiseman,DJ
AU - Laponogov,I
AU - Donaldson,G
AU - Wedzicha,JA
AU - Johnston,SL
AU - Singanayagam,A
AU - Hanna,GB
DO - 10.1164/rccm.202103-0660OC
EP - 1085
PY - 2021///
SN - 1073-449X
SP - 1075
TI - Virus-induced volatile organic compounds are detectable in exhaled breath during pulmonary infection.
T2 - American Journal of Respiratory and Critical Care Medicine
UR - http://dx.doi.org/10.1164/rccm.202103-0660OC
UR - https://www.ncbi.nlm.nih.gov/pubmed/34319857
UR - https://www.atsjournals.org/doi/10.1164/rccm.202103-0660OC
UR - http://hdl.handle.net/10044/1/91220
VL - 204
ER -
