@article{Mullineaux:2021:10.1128/mBio.02410-21,
author = {Mullineaux, Sanders C and Carson, D and Hopkins, E and Glegola-Madejska, I and Escobar-Zepeda, A and Browne, H and Lawley, T and Frankel, G},
doi = {10.1128/mBio.02410-21},
journal = {mBio},
pages = {1--19},
title = {Citrobacter amalonaticus inhibits the growth of Citrobacter rodentium in the gut lumen},
url = {http://dx.doi.org/10.1128/mBio.02410-21},
volume = {5},
year = {2021}
}

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TY  - JOUR
AB  - The gut microbiota plays a crucial role in susceptibility to enteric pathogens, including Citrobacter rodentium, a model extracellular mouse pathogen that colonizes the colonic mucosa. C. rodentium infection outcomes vary between mouse strains, with C57BL/6 and C3H/HeN mice clearing or succumbing to the infection respectively. Kanamycin (Kan) treatment at the peak of C57BL/6 mouse infection with Kan-resistant C. rodentium resulted in re-localisation of the pathogen from the colonic mucosa and cecum to solely the cecal luminal contents; cessation of the Kan treatment resulted in rapid clearance of the pathogen. We now show that in C3H/HeN mice, following Kan-induced displacement of C. rodentium to the cecum, the pathogen stably colonizes the cecal lumen of 65% of the mice in the absence of continued antibiotic treatment, a phenomenon we term antibiotic-induced bacterial commensalisation (AIBC). AIBC C. rodentium was well-tolerated by the host, which showed little signs of inflammation; passaged AIBC C. rodentium robustly infected naïve C3H/HeN mice suggesting that the AIBC state is transient and did not select for genetically avirulent C. rodentium mutants. Following withdrawal of antibiotic treatment, 35% of C3H/HeN mice were able to prevent C. rodentium commensalisation in the gut lumen. These mice presented a bloom of a commensal species, Citrobacter amalonaticus, which inhibited the growth of C. rodentium in vitro in a contact-dependant manner, and luminal growth of AIBC C. rodentium in vivo. Overall our data suggest that commensal species can confer colonization resistance against closely-related pathogenic species.
AU  - Mullineaux,Sanders C
AU  - Carson,D
AU  - Hopkins,E
AU  - Glegola-Madejska,I
AU  - Escobar-Zepeda,A
AU  - Browne,H
AU  - Lawley,T
AU  - Frankel,G
DO  - 10.1128/mBio.02410-21
EP  - 19
PY  - 2021///
SN  - 2150-7511
SP  - 1
TI  - Citrobacter amalonaticus inhibits the growth of Citrobacter rodentium in the gut lumen
T2  - mBio
UR  - http://dx.doi.org/10.1128/mBio.02410-21
UR  - https://journals.asm.org/doi/10.1128/mBio.02410-21
UR  - http://hdl.handle.net/10044/1/91671
VL  - 5
ER  - 

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