Citation

BibTex format

@article{Mullineaux:2022:10.1128/mbio.03233-21,
author = {Mullineaux, Sanders C and Kozik, Z and Sanchez, Garrido J and Hopkins, EGD and Choudhary, JS and Frankel, G},
doi = {10.1128/mbio.03233-21},
journal = {mBio},
pages = {1--18},
title = {Citrobacter rodentium infection induces persistent molecular changes and interferon gamma-dependent major histocompatibility complex class II expression in the colonic epithelium},
url = {http://dx.doi.org/10.1128/mbio.03233-21},
volume = {13},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Most studies of infections at mucosal surfaces have focused on the acute phase of the disease. Consequently, little is known about the molecular processes that underpin tissue recovery and the long-term consequences postinfection. Here, we conducted temporal deep quantitative proteomic analysis of colonic intestinal epithelial cells (cIECs) from mice infected with the natural mouse pathogen Citrobacter rodentium over time points corresponding to the late steady-state phase (10 days postinfection [DPI]), the clearance phase (13 to 20 DPI), and 4 weeks after the pathogen has been cleared (48 DPI). C. rodentium, which relies on a type III secretion system to infect, is used to model infections with enteropathogenic and enterohemorrhagic Escherichia coli. We observe a strong upregulation of inflammatory signaling and nutritional immunity responses during the clearance phase of the infection. Despite morphological tissue recovery, chromogranin B (ChgB)-positive endocrine cells remained significantly below baseline levels at 48 DPI. In contrast, we observed an increased abundance of proteins involved in antigen processing and presentation 4 weeks after pathogen clearance. In particular, long-term changes were characterized by a persistent interferon gamma (IFN-γ) response and the expression of major histocompatibility complex class II (MHCII) molecules in 60% of the EpCAM+ cIECs, which were not seen in Ifnγ−/− mice. Nonetheless, both wild-type and Ifnγ−/− mice mounted similar systemic and colonic IgG responses to C. rodentium and were equally protected from rechallenge, suggesting that cIEC MHCII is not necessary for protective immunity against C. rodentium.
AU  - Mullineaux,Sanders C
AU  - Kozik,Z
AU  - Sanchez,Garrido J
AU  - Hopkins,EGD
AU  - Choudhary,JS
AU  - Frankel,G
DO  - 10.1128/mbio.03233-21
EP  - 18
PY  - 2022///
SN  - 2150-7511
SP  - 1
TI  - Citrobacter rodentium infection induces persistent molecular changes and interferon gamma-dependent major histocompatibility complex class II expression in the colonic epithelium
T2  - mBio
UR  - http://dx.doi.org/10.1128/mbio.03233-21
UR  - https://journals.asm.org/doi/full/10.1128/mbio.03233-21
UR  - http://hdl.handle.net/10044/1/94073
VL  - 13
ER  -
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