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  • Journal article
    Moscoso JA, Jaeger T, Valentini M, Hui K, Jenal U, Filloux Aet al., 2014,

    The Diguanylate Cyclase SadC Is a Central Player in Gac/Rsm-Mediated Biofilm Formation in Pseudomonas aeruginosa

    , Journal of Bacteriology, Vol: 196, Pages: 4081-4088, ISSN: 1098-5530

    Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen and a threat for immunocompromised and cysticfibrosis patients. It is responsible for acute and chronic infections and can switch between these lifestyles upon taking an informeddecision involving complex regulatory networks. The RetS/LadS/Gac/Rsm network and the cyclic-di-GMP (c-di-GMP)signaling pathways are both central to this phenomenon redirecting the P. aeruginosa population toward a biofilm mode ofgrowth, which is associated with chronic infections. While these two pathways were traditionally studied independently fromeach other, we recently showed that cellular levels of c-di-GMP are increased in the hyperbiofilm retS mutant. Here, we have formallyestablished the link between the two networks by showing that the SadC diguanylate cyclase is central to the Gac/Rsmassociatedphenotypes, notably, biofilm formation. Importantly, SadC is involved in the signaling that converges onto the RsmAtranslational repressor either via RetS/LadS or via HptB/HsbR. Although the level of expression of the sadC gene does not seemto be impacted by the regulatory cascade, the production of the SadC protein is tightly repressed by RsmA. This adds to thegrowing complexity of the signaling network associated with c-di-GMP in P. aeruginosa. While this organism possesses morethan 40 c-di-GMP-related enzymes, it remains unclear how signaling specificity is maintained within the c-di-GMP network. Thefinding that SadC but no other diguanylate cyclase is related to the formation of biofilm governed by the Gac/Rsm pathway furthercontributes to understanding of this insulation mechanism.
  • Journal article
    Matsuo E, Leon E, Matthews SJ, Roy Pet al., 2014,

    Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV

    , BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 451, Pages: 603-608, ISSN: 0006-291X
  • Journal article
    Brown DR, Barton G, Pan Z, Buck M, Wigneshwerarajet al., 2014,

    Combinatorial stress responses: direct coupling of two major stress responses in Escherichia coli

    , Microbial Cell, Vol: 1, Pages: 315-317, ISSN: 2311-2638

    Nitrogen is an essential element for all life, and this isno different for the bacterial cell. Numerous cellularmacromolecules contain nitrogen, including proteins,nucleic acids and cell wall components. In Escherichiacoli and related bacteria, the nitrogen stress (Ntr) responseallows cells to rapidly sense and adapt to nitrogenlimitation by scavenging for alternative nitrogensources through the transcriptional activation oftransport systems and catabolic and biosynthetic operonsby the global transcriptional regulator NtrC. Nitrogen-starvedbacterial cells also synthesize the(p)ppGpp effector molecules of a second global bacterialstress response - the stringent response. Recently,we showed that the transcription of relA, the genewhich encodes the major (p)ppGpp synthetase in E.coli, is activated by NtrC during nitrogen starvation.Our results revealed that in E. coli and related bacteria,NtrC functions in combinatorial stress and serves tocouple two major stress responses, the Ntr responseand stringent response.
  • Conference paper
    Mota J, Holden DW, Domingues L, 2014,

    The <i>Salmonella</i> effector SteA contributes to the control of membrane dynamics of <i>Salmonella</i>-containing vacuoles

    , FEBS EMBO 2014 Conference, Publisher: WILEY-BLACKWELL, Pages: 766-767, ISSN: 1742-464X
  • Journal article
    Berry AA, Yang Y, Pakharukova N, Garnett JA, Lee W-C, Cota E, Marchant J, Roy S, Tuittila M, Liu B, Inman KG, Ruiz-Perez F, Mandomando I, Nataro JP, Zavialov AV, Matthews Set al., 2014,

    Structural Insight into Host Recognition by Aggregative Adherence Fimbriae of Enteroaggregative <i>Escherichia coli</i>

    , PLOS PATHOGENS, Vol: 10, ISSN: 1553-7366
  • Journal article
    Collins JW, Keeney KM, Crepin VE, Rathinam VAK, Fitzgerald KA, Finlay BB, Frankel Get al., 2014,

    <i>Citrobacter rodentium</i>: infection, inflammation and the microbiota

    , NATURE REVIEWS MICROBIOLOGY, Vol: 12, Pages: 612-623, ISSN: 1740-1526
  • Journal article
    Mousnier A, Schroeder GN, Stoneham CA, So EC, Garnett JA, Yu L, Matthews SJ, Choudhary JS, Hartland EL, Frankel Get al., 2014,

    A New Method To Determine <i>In Vivo</i> Interactomes Reveals Binding of the <i>Legionella pneumophila</i> Effector PieE to Multiple Rab GTPases

    , MBIO, Vol: 5, ISSN: 2150-7511
  • Journal article
    Gründling A, 2014,

    Milestones in nucleotide signaling research: Nucleotide signals are found in bacteria as well as eukaryotes, and may act intra- or extracellularly

    , Microbe, Vol: 9, Pages: 315-320, ISSN: 1558-7452
  • Journal article
    Stevens MP, Frankel GM, 2014,

    The Locus of Enterocyte Effacement and Associated Virulence Factors of Enterohemorrhagic Escherichia coli.

    , Microbiol Spectr, Vol: 2

    A subset of Shiga toxin-producing Escherichia coli strains, termed enterohemorrhagic E. coli (EHEC), is defined in part by the ability to produce attaching and effacing (A/E) lesions on intestinal epithelia. Such lesions are characterized by intimate bacterial attachment to the apical surface of enterocytes, cytoskeletal rearrangements beneath adherent bacteria, and destruction of proximal microvilli. A/E lesion formation requires the locus of enterocyte effacement (LEE), which encodes a Type III secretion system that injects bacterial proteins into host cells. The translocated proteins, termed effectors, subvert a plethora of cellular pathways to the benefit of the pathogen, for example, by recruiting cytoskeletal proteins, disrupting epithelial barrier integrity, and interfering with the induction of inflammation, phagocytosis, and apoptosis. The LEE and selected effectors play pivotal roles in intestinal persistence and virulence of EHEC, and it is becoming clear that effectors may act in redundant, synergistic, and antagonistic ways during infection. Vaccines that target the function of the Type III secretion system limit colonization of reservoir hosts by EHEC and may thus aid control of zoonotic infections. Here we review the features and functions of the LEE-encoded Type III secretion system and associated effectors of E. coli O157:H7 and other Shiga toxin-producing E. coli strains.
  • Journal article
    Baek KT, Grundling A, Mogensen RG, Thogersen L, Petersen A, Paulander W, Frees Det al., 2014,

    β-Lactam Resistance in Methicillin-Resistant <i>Staphylococcus aureus</i> USA300 Is Increased by Inactivation of the ClpXP Protease

    , ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol: 58, Pages: 4593-4603, ISSN: 0066-4804

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.

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