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Journal articleLiu B, Shadrin A, Sheppard C, et al., 2014,
A bacteriophage transcription regulator inhibits bacterial transcription initiation by Sigma-factor displacement
, Nucleic Acids Research, Vol: 42, Pages: 4294-4305, ISSN: 0305-1048Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic β and β’ subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the σ70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.
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Journal articleSharma A, Leach RN, Gell C, et al., 2014,
Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies
, NUCLEIC ACIDS RESEARCH, Vol: 42, Pages: 5177-5190, ISSN: 0305-1048- Author Web Link
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- Citations: 18
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Journal articleReichmann NT, Cassona CP, Monteiro JM, et al., 2014,
Differential localization of LTA synthesis proteins and their interaction with the cell division machinery in <i>Staphylococcus aureus</i>
, MOLECULAR MICROBIOLOGY, Vol: 92, Pages: 273-286, ISSN: 0950-382X- Author Web Link
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- Citations: 36
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Journal articleBall G, Filloux A, Voulhoux R, 2014,
A method to capture large DNA fragments from genomic DNA.
, Methods Mol Biol, Vol: 1149, Pages: 491-500The gene capture technique is a powerful tool that allows the cloning of large DNA regions (up to 80 kb), such as entire genomic islands, without using restriction enzymes or DNA amplification. This technique takes advantage of the high recombinant capacity of the yeast. A "capture" vector containing both ends of the target DNA region must first be constructed. The target region is then captured by co-transformation and recombination in yeast between the "capture" vector and appropriate genomic DNA. The selected recombinant plasmid can be verified by sequencing and transferred in the bacteria for multiple applications. This chapter describes a protocol specifically adapted for Pseudomonas aeruginosa genomic DNA capture.
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Journal articleFagan RP, Fairweather NF, 2014,
Biogenesis and functions of bacterial S-layers
, NATURE REVIEWS MICROBIOLOGY, Vol: 12, Pages: 211-222, ISSN: 1740-1526- Author Web Link
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- Citations: 226
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Journal articleWlodarska M, Thaiss CA, Nowarski R, et al., 2014,
NLRP6 Inflammasome Orchestrates the Colonic Host-Microbial Interface by Regulating Goblet Cell Mucus Secretion
, CELL, Vol: 156, Pages: 1045-1059, ISSN: 0092-8674- Author Web Link
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- Citations: 487
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Journal articleDionne MS, 2014,
Immune-metabolic interaction in <i>Drosophila</i>
, FLY, Vol: 8, Pages: 75-79, ISSN: 1933-6934- Author Web Link
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- Citations: 21
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Journal articleGouzy A, Larrouy-Maumus G, Bottai D, et al., 2014,
<i>Mycobacterium tuberculosis</i> Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection
, PLOS PATHOGENS, Vol: 10, ISSN: 1553-7366- Author Web Link
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- Citations: 114
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Journal articleRudkin JK, Laabei M, Edwards AM, et al., 2014,
Oxacillin Alters the Toxin Expression Profile of Community-Associated Methicillin-Resistant <i>Staphylococcus aureus</i>
, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol: 58, Pages: 1100-1107, ISSN: 0066-4804- Author Web Link
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- Citations: 44
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Journal articleSampson SL, Saraiva L, Gustafsson K, et al., 2014,
Cell Electrospinning: An In Vitro and In Vivo Study
, Small, Vol: 10, Pages: 78-82, ISSN: 1613-6810Cell electrospinning and aerodynamically assisted bio-threading are novel bioplatforms for directly forming large quantities of cell-laden scaffolds for creating living sheets and vessels in three-dimensions. The functional biological architectures generated will be useful in both the laboratory and the clinic.
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