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Journal articleLai Y, Rosenshine I, Leong JM, et al., 2013,
Intimate host attachment: enteropathogenic and enterohaemorrhagic <i>Escherichia coli</i>
, CELLULAR MICROBIOLOGY, Vol: 15, Pages: 1796-1808, ISSN: 1462-5814- Author Web Link
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- Citations: 81
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Journal articleMercedes Palomino M, Allievi MC, Gruendling A, et al., 2013,
Osmotic stress adaptation in <i>Lactobacillus casei</i> BL23 leads to structural changes in the cell wall polymer lipoteichoic acid
, MICROBIOLOGY-SGM, Vol: 159, Pages: 2416-2426, ISSN: 1350-0872- Author Web Link
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- Citations: 34
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Journal articleHarding CR, Mattheis C, Mousnier A, et al., 2013,
LtpD Is a Novel <i>Legionella pneumophila</i> Effector That Binds Phosphatidylinositol 3-Phosphate and Inositol Monophosphatase IMPA1
, INFECTION AND IMMUNITY, Vol: 81, Pages: 4261-4270, ISSN: 0019-9567- Author Web Link
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- Citations: 28
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Journal articleClark RI, Tan SWS, Pean CB, et al., 2013,
MEF2 Is an In Vivo Immune-Metabolic Switch
, CELL, Vol: 155, Pages: 435-447, ISSN: 0092-8674- Author Web Link
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- Citations: 114
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Journal articleDionne MS, 2013,
Comparative immunology: allorecognition and variable surface receptors outside the jawed vertebrates
, CURRENT OPINION IN IMMUNOLOGY, Vol: 25, Pages: 608-612, ISSN: 0952-7915- Author Web Link
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- Citations: 3
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Journal articleTulli L, Marchi S, Petracca R, et al., 2013,
CbpA: a novel surface exposed adhesin of <i>Clostridium difficile</i> targeting human collagen
, CELLULAR MICROBIOLOGY, Vol: 15, Pages: 1674-1687, ISSN: 1462-5814- Author Web Link
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- Citations: 44
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Journal articlePearson JS, Giogha C, Ong SY, et al., 2013,
A type III effector antagonizes death receptor signalling during bacterial gut infection
, NATURE, Vol: 501, Pages: 247-+, ISSN: 0028-0836- Author Web Link
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- Citations: 206
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Journal articleAndreu N, Zelmer A, Sampson SL, et al., 2013,
Rapid in vivo assessment of drug efficacy against Mycobacterium tuberculosis using an improved firefly luciferase
, Journal of Antimicrobial Chemotherapy, Vol: 68, Pages: 2118-2127, ISSN: 1460-2091Objectives In vivo experimentation is costly and time-consuming, and presents a major bottleneck in anti-tuberculosis drug development. Conventional methods rely on the enumeration of bacterial colonies, and it can take up to 4 weeks for Mycobacterium tuberculosis to grow on agar plates. Light produced by recombinant bacteria expressing luciferase enzymes can be used as a marker of bacterial load, and disease progression can be easily followed non-invasively in live animals by using the appropriate imaging equipment. The objective of this work was to develop a bioluminescence-based mouse model of tuberculosis to assess antibiotic efficacy against M. tuberculosis in vivo.Methods We used an M. tuberculosis strain carrying a red-shifted derivative of the firefly luciferase gene (FFlucRT) to infect mice, and monitored disease progression in living animals by bioluminescence imaging before and after treatment with the frontline anti-tuberculosis drug isoniazid. The resulting images were analysed and the bioluminescence was correlated with bacterial counts.Results Using bioluminescence imaging we detected as few as 1.7 × 103 and 7.5 × 104 reporter bacteria ex vivo and in vivo, respectively, in the lungs of mice. A good correlation was found between bioluminescence and bacterial load in both cases. Furthermore, a marked reduction in luminescence was observed in living mice given isoniazid treatment.Conclusions We have shown that an improved bioluminescent strain of M. tuberculosis can be visualized by non-invasive imaging in live mice during an acute, progressive infection and that this technique can be used to rapidly visualize and quantify the effect of antibiotic treatment. We believe that the model presented here will be of great benefit in early drug discovery as an easy and rapid way to identify active compounds in vivo.
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Journal articleReichmann NT, Cassona CP, Gruendling A, 2013,
Revised mechanism of D-alanine incorporation into cell wall polymers in Gram-positive bacteria
, MICROBIOLOGY-SGM, Vol: 159, Pages: 1868-1877, ISSN: 1350-0872- Author Web Link
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- Citations: 69
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Journal articleFilloux A, 2013,
MICROBIOLOGY A weapon for bacterial warfare
, NATURE, Vol: 500, Pages: 284-285, ISSN: 0028-0836- Author Web Link
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- Citations: 7
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