BibTex format
@article{Al:2015:infdis/jiv076,
author = {Al, Shammari B and Shiomi, T and Tezera, L and Bielecka, MK and Workman, V and Sathyamoorthy, T and Mauri, F and Jayasinghe, SN and Robertson, BD and D'Armiento, J and Friedland, JS and Elkington, PT},
doi = {infdis/jiv076},
journal = {Journal of Infectious Diseases},
pages = {463--473},
title = {The extracellular matrix regulates granuloma necrosis in tuberculosis},
url = {http://dx.doi.org/10.1093/infdis/jiv076},
volume = {212},
year = {2015}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - A central tenet of tuberculosis pathogenesis is that caseous necrosis leads to extracellular matrix destruction and bacterial transmission. We reconsider the underlying mechanism of tuberculosis pathology and demonstrate that collagen destruction may be a critical initial event, causing caseous necrosis as opposed to resulting from it. In human tuberculosis granulomas, regions of extracellular matrix destruction map to areas of caseous necrosis. In mice, transgenic expression of human matrix metalloproteinase 1 causes caseous necrosis, the pathological hallmark of human tuberculosis. Collagen destruction is the principal pathological difference between humanised mice and wild-type mice with tuberculosis, whereas the release of proinflammatory cytokines does not differ, demonstrating that collagen breakdown may lead to cell death and caseation. To investigate this hypothesis, we developed a 3-dimensional cell culture model of tuberculosis granuloma formation, using bioelectrospray technology. Collagen improved survival of Mycobacterium tuberculosis–infected cells analyzed on the basis of a lactate dehydrogenase release assay, propidium iodide staining, and measurement of the total number of viable cells. Taken together, these findings suggest that collagen destruction is an initial event in tuberculosis immunopathology, leading to caseous necrosis and compromising the immune response, revealing a previously unappreciated role for the extracellular matrix in regulating the host-pathogen interaction.
AU - Al,Shammari B
AU - Shiomi,T
AU - Tezera,L
AU - Bielecka,MK
AU - Workman,V
AU - Sathyamoorthy,T
AU - Mauri,F
AU - Jayasinghe,SN
AU - Robertson,BD
AU - D'Armiento,J
AU - Friedland,JS
AU - Elkington,PT
DO - infdis/jiv076
EP - 473
PY - 2015///
SN - 1537-6613
SP - 463
TI - The extracellular matrix regulates granuloma necrosis in tuberculosis
T2 - Journal of Infectious Diseases
UR - http://dx.doi.org/10.1093/infdis/jiv076
UR - https://academic.oup.com/jid/article/212/3/463/854384
UR - http://hdl.handle.net/10044/1/39614
VL - 212
ER -
