In this section
@article{Aragon:2015:10.1111/1462-2920.12856,
author = {Aragon, IM and Pérez-Mendoza, D and Moscoso, JA and Faure, E and Guery, B and Gallegos, M-T and Filloux, A and Ramos, C},
doi = {10.1111/1462-2920.12856},
journal = {Environmental Microbiology},
pages = {4332--4351},
title = {Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections},
url = {http://dx.doi.org/10.1111/1462-2920.12856},
volume = {17},
year = {2015}
}
TY - JOUR
AB - The second messenger cyclic di-GMP (c-di-GMP) controls the transition between different lifestyles in bacterial pathogens. Here, we report the identification of DgcP (diguanylate cyclase conserved in Pseudomonads), whose activity in the olive tree pathogen Pseudomonas savastanoi pv. savastanoi is dependent on the integrity of its GGDEF domain. Furthermore, deletion of the dgcP gene revealed that DgcP negatively regulates motility and positively controls biofilm formation in both the olive tree pathogen P. savastanoi pv. savastanoi and the human opportunistic pathogen Pseudomonas aeruginosa. Overexpression of the dgcP gene in P. aeruginosaPAK led to increased exopolysaccharide production and upregulation of the type VI secretion system; in turn, it repressed the type III secretion system, which is a hallmark of chronic infections and persistence for P. aeruginosa. Deletion of the dgcP gene in P. savastanoi pv. savastanoi NCPPB 3335 and P. aeruginosaPAK reduced their virulence in olive plants and in a mouse acute lung injury model respectively. Our results show that diguanylate cyclase DgcP is a conserved Pseudomonas protein with a role in virulence, and confirm the existence of common c-di-GMP signalling pathways that are capable of regulating plant and human Pseudomonas spp. infections.
AU - Aragon,IM
AU - Pérez-Mendoza,D
AU - Moscoso,JA
AU - Faure,E
AU - Guery,B
AU - Gallegos,M-T
AU - Filloux,A
AU - Ramos,C
DO - 10.1111/1462-2920.12856
EP - 4351
PY - 2015///
SN - 1462-2920
SP - 4332
TI - Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections
T2 - Environmental Microbiology
UR - http://dx.doi.org/10.1111/1462-2920.12856
UR - http://hdl.handle.net/10044/1/21710
VL - 17
ER -
CBRB
Imperial College London
Flowers Building
Exhibition Road
London SW7 2AZ
