@article{Armstrong-James:2016:10.1164/rccm.201601-0070OC,
author = {Armstrong-James, DPH},
doi = {10.1164/rccm.201601-0070OC},
journal = {American Journal of Respiratory and Critical Care Medicine},
pages = {1127--1139},
title = {Calcineurin Orchestrates Lateral Transfer of Aspergillus fumigatus during Macrophage Cell Death},
url = {http://dx.doi.org/10.1164/rccm.201601-0070OC},
volume = {194},
year = {2016}
}

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TY  - JOUR
AB  - Rationale: Pulmonary aspergillosis is a lethal mold infection in the immunocompromised host. Understanding initial control of infection and how this is altered in the immunocompromised host are key goals for comprehension of the pathogenesis of pulmonary aspergillosis.Objectives: To characterize the outcome of human macrophage infection with Aspergillus fumigatus and how this is altered in transplant recipients on calcineurin inhibitor immunosuppressants.Methods: We defined the outcome of human macrophage infection with A. fumigatus, as well as the impact of calcineurin inhibitors, through a combination of single-cell fluorescence imaging, transcriptomics, proteomics, and in vivo studies.Measurements and Main Results: Macrophage phagocytosis of A. fumigatus enabled control of 90% of fungal germination. However, fungal germination in the late phagosome led to macrophage necrosis. During programmed necroptosis, we observed frequent cell–cell transfer of A. fumigatus between macrophages, which assists subsequent control of germination in recipient macrophages. Lateral transfer occurred through actin-dependent exocytosis of the late endosome in a vasodilator-stimulated phosphoprotein envelope. Its relevance to the control of fungal germination was also shown by direct visualization in our zebrafish aspergillosis model in vivo. The calcineurin inhibitor FK506 (tacrolimus) reduced cell death and lateral transfer in vitro by 50%. This resulted in uncontrolled fungal germination in macrophages and also resulted in hyphal escape.Conclusions: These observations identify programmed, necrosis-dependent lateral transfer of A. fumigatus between macrophages as an important host strategy for controlling fungal germination. This process is critically dependent on calcineurin. Our studies provide fundamental insights into the pathogenesis of pulmonary aspergillosis in the immunocompromised host.
AU  - Armstrong-James,DPH
DO  - 10.1164/rccm.201601-0070OC
EP  - 1139
PY  - 2016///
SN  - 1535-4970
SP  - 1127
TI  - Calcineurin Orchestrates Lateral Transfer of Aspergillus fumigatus during Macrophage Cell Death
T2  - American Journal of Respiratory and Critical Care Medicine
UR  - http://dx.doi.org/10.1164/rccm.201601-0070OC
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27163634
UR  - http://hdl.handle.net/10044/1/33585
VL  - 194
ER  - 

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