@article{Goddard:2016:10.1111/mmi.13546,
author = {Goddard, AD and Bali, S and Mavridou, DAI and Luque-, Almagro VM and Gates, AJ and Roldán, MD and Newstead, S and Richardson, DJ and Ferguson, SJ},
doi = {10.1111/mmi.13546},
journal = {Molecular Microbiology},
pages = {117--133},
title = {The Paracoccus denitrificans NarK-like nitrate and nitrite transporters; probing nitrate uptake and nitrate/nitrite exchange mechanisms},
url = {http://dx.doi.org/10.1111/mmi.13546},
volume = {103},
year = {2016}
}

Download

TY  - JOUR
AB  - Nitrate and nitrite transport across biological membranes is often facilitated by protein transporters  that  are  members  of  the  major  facilitator  superfamily. Paracoccus denitrificans  contains  an  unusual  arrangement  whereby  two  of  these  transporters, NarK1 and NarK2, are fused into a single protein, NarK, which delivers nitrate to the respiratory  nitrate  reductase  and  transfers  the  product,  nitrite,  to  the  periplasm.  Our complementation  studies,  using  a  mutant  lacking  the  nitrate/proton  symporter  NasA from  the  assimilatory  nitrate  reductase  pathway,  support  that  NarK1  functions  as  a nitrate/proton symporter while NarK2 is a nitrate/nitrite antiporter. Through the same experimental  system, we  find  that Escherichia coli  NarK and  NarU can  complement deletions in both narK and nasA in P. denitrificans, suggesting that, while these proteins are most likely nitrate/nitrite antiporters, they can also act in the net uptake of nitrate. Finally, we argue that primary sequence analysis and structural modelling do not readily explain why NasA, NarK1 and NarK2, as well as other transporters from this protein family, have such different functions, ranging from net nitrate uptake to nitrate/nitrite exchange.
AU  - Goddard,AD
AU  - Bali,S
AU  - Mavridou,DAI
AU  - Luque-,Almagro VM
AU  - Gates,AJ
AU  - Roldán,MD
AU  - Newstead,S
AU  - Richardson,DJ
AU  - Ferguson,SJ
DO  - 10.1111/mmi.13546
EP  - 133
PY  - 2016///
SN  - 1365-2958
SP  - 117
TI  - The Paracoccus denitrificans NarK-like nitrate and nitrite transporters; probing nitrate uptake and nitrate/nitrite exchange mechanisms
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/mmi.13546
UR  - http://hdl.handle.net/10044/1/41270
VL  - 103
ER  - 

Download