@article{Tabib-Salazar:2017:nar/gkx370,
author = {Tabib-Salazar, A and Liu, B and Shadrin, A and Burchell, L and Wang, Z and Wang, Z and Goren, MG and Yosef, I and Qimron, U and Severinov, K and Matthews, SJ and Wigneshweraraj, S},
doi = {nar/gkx370},
journal = {Nucleic Acids Research},
pages = {7697--7707},
title = {Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein},
url = {http://dx.doi.org/10.1093/nar/gkx370},
volume = {45},
year = {2017}
}

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TY  - JOUR
AB  - Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the bacterial RNA polymerase (RNAP) by the 7 kDa T7 protein Gp2. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.7, which adopts a winged helix-turn-helix-like structure and specifically represses transcription initiation from host RNAP-dependent promoters on the phage genome via a mechanism that involves interaction with DNA and the bacterial RNAP. Whereas Gp2 is indispensable for T7 growth in E. coli, we show that Gp5.7 is required for optimal infection outcome. Our findings provide novel insights into how phages fine-tune the activity of the host transcription machinery to ensure both successful and efficient phage progeny development.
AU  - Tabib-Salazar,A
AU  - Liu,B
AU  - Shadrin,A
AU  - Burchell,L
AU  - Wang,Z
AU  - Wang,Z
AU  - Goren,MG
AU  - Yosef,I
AU  - Qimron,U
AU  - Severinov,K
AU  - Matthews,SJ
AU  - Wigneshweraraj,S
DO  - nar/gkx370
EP  - 7707
PY  - 2017///
SN  - 1362-4962
SP  - 7697
TI  - Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gkx370
UR  - http://hdl.handle.net/10044/1/48259
VL  - 45
ER  - 

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