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@article{McCarthy:2017:10.1007/978-1-4939-7240-1_17,
author = {McCarthy, RR and Valentini, M and Filloux, A},
doi = {10.1007/978-1-4939-7240-1_17},
journal = {Methods Mol Biol},
pages = {213--224},
title = {Contribution of Cyclic di-GMP in the Control of Type III and Type VI Secretion in Pseudomonas aeruginosa.},
url = {http://dx.doi.org/10.1007/978-1-4939-7240-1_17},
volume = {1657},
year = {2017}
}
TY - JOUR
AB - Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems.
AU - McCarthy,RR
AU - Valentini,M
AU - Filloux,A
DO - 10.1007/978-1-4939-7240-1_17
EP - 224
PY - 2017///
SP - 213
TI - Contribution of Cyclic di-GMP in the Control of Type III and Type VI Secretion in Pseudomonas aeruginosa.
T2 - Methods Mol Biol
UR - http://dx.doi.org/10.1007/978-1-4939-7240-1_17
UR - https://www.ncbi.nlm.nih.gov/pubmed/28889297
VL - 1657
ER -
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