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@article{Wen:2017:10.1021/acssynbio.7b00219,
author = {Wen, KY and Cameron, L and Chappell, J and Jensen, K and Bell, DJ and Kelwick, R and Kopniczky, M and Davies, JC and Filloux, A and Freemont, PS},
doi = {10.1021/acssynbio.7b00219},
journal = {ACS Synthetic Biology},
pages = {2293--2301},
title = {A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.},
url = {http://dx.doi.org/10.1021/acssynbio.7b00219},
volume = {6},
year = {2017}
}

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TY  - JOUR
AB  - Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA-encoded biosensor in cell-free protein expression systems can be used to measure a bacterial biomarker of Pseudomonas aeruginosa infection from human sputum samples. By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC-MS measurements of the same samples. This study further illustrates the potential of modular cell-free biosensors as rapid, low-cost detection assays that can inform clinical practice.
AU  - Wen,KY
AU  - Cameron,L
AU  - Chappell,J
AU  - Jensen,K
AU  - Bell,DJ
AU  - Kelwick,R
AU  - Kopniczky,M
AU  - Davies,JC
AU  - Filloux,A
AU  - Freemont,PS
DO  - 10.1021/acssynbio.7b00219
EP  - 2301
PY  - 2017///
SN  - 2161-5063
SP  - 2293
TI  - A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.
T2  - ACS Synthetic Biology
UR  - http://dx.doi.org/10.1021/acssynbio.7b00219
UR  - http://hdl.handle.net/10044/1/53616
VL  - 6
ER  -

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