In this section
@article{Abbs:1993:10.1016/0966-3274(93)90058-g,
author = {Abbs, IC and Pratt, JR and Dallman, MJ and Sacks, SH},
doi = {10.1016/0966-3274(93)90058-g},
journal = {Transpl Immunol},
pages = {45--51},
title = {Analysis of activated T cell infiltrates in rat renal allografts by gamma camera imaging after injection of 123iodine-interleukin 2.},
url = {http://dx.doi.org/10.1016/0966-3274(93)90058-g},
volume = {1},
year = {1993}
}
TY - JOUR
AB - Activated T cells bearing receptors for interleukin 2 (IL-2) play an important role in immunity and in immunopathological processes such as allograft rejection. In order to investigate the presence of activated T cells in lymphocytic infiltrates in transplanted kidneys, we investigated the uptake and retention of radioactivity after an intravenous injection of radioiodinated IL-2 in experimentally transplanted rats. IL-2 was enzyme radiolabelled with 123iodine using a glucose oxidase/lactoperoxidase method and shown to retain specific binding on an IL-2 receptor positive cell line, C58E6. To examine the kinetics of 123iodine-interleukin 2 (123I-IL-2) uptake in vivo, animals that had been transplanted five days previously with allogeneic or syngeneic grafts were injected with 123I-IL-2 and then imaged using an external gamma camera. Radioactivity was measured at time points up to 240 min after intravenous injection of 123I-IL-2. Four groups of animals were examined: allogeneic grafts (n = 7); syngeneic grafts (n = 6); ischaemic native kidneys (n = 5) all following injection with 123I-IL-2; and allogeneic transplants (n = 5) after injection of 123I-lactalbumin, an irrelevant molecule of similar molecular weight to IL-2. The peak radioactivity after injection was measured and the amount of radioactivity retained in the graft at increasing time intervals after injection was expressed as a function of initial peak radioactivity. At four hours after injection of 123I-IL-2, mean retention of activity by rejecting grafts was 77(+/- 2.68)% of peak activity, compared to 45(+/- 6.38)% in syngeneic controls (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
AU - Abbs,IC
AU - Pratt,JR
AU - Dallman,MJ
AU - Sacks,SH
DO - 10.1016/0966-3274(93)90058-g
EP - 51
PY - 1993///
SN - 0966-3274
SP - 45
TI - Analysis of activated T cell infiltrates in rat renal allografts by gamma camera imaging after injection of 123iodine-interleukin 2.
T2 - Transpl Immunol
UR - http://dx.doi.org/10.1016/0966-3274(93)90058-g
UR - https://www.ncbi.nlm.nih.gov/pubmed/8081762
VL - 1
ER -