In this section
@article{Guo:2001:10.1053/jpsu.2001.20715,
author = {Guo, WH and Tian, L and Chan, KL and Dallman, M and Tam, PK},
doi = {10.1053/jpsu.2001.20715},
journal = {J Pediatr Surg},
pages = {352--356},
title = {Role of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft.},
url = {http://dx.doi.org/10.1053/jpsu.2001.20715},
volume = {36},
year = {2001}
}
TY - JOUR
AB - BACKGROUND/PURPOSE: Results of small bowel transplantation remain unsatisfactory because of severe immune rejection. The current study aims to elucidate the role of activation of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft and, hence, provide the immunologic basis for developing new therapeutic strategies. METHODS: We used an MHC fully mismatched (DA to Lewis) heterotopic rat small bowel transplant model and a unique FK506-based immunosuppressive regimen, which suppresses early acute rejection but does not prevent late acute rejection. Flow cytometric analysis was used to quantitate the number of activated CD4+ and CD8+ T cells in graft and host mesenteric lymph nodes. RESULTS: The survival (mean +/- SD) of intestinal allograft was significantly prolonged, from 6.6 +/- 0.84 days for the untreated group to 40.7 +/- 14.1 days for the FK506-treated group. Activation of CD4+ cells was suppressed significantly in the FK506-treated group on postoperative day 7 compared with the untreated group (29.4% +/- 3.55% v 52.83% +/- 11.9%; P <.01). Activation of CD8+ cells was similarly suppressed (31.5 +/- 10.34% v 48.53 +/- 14.34%; P <.05). Interestingly, at late acute rejection, activated CD4+ and CD8+ T cells remained at almost the same low levels as those on postoperative day 7 in the FK506-treated group. The spleen to body weight ratio was significantly increased in the untreated group (0.53 +/- 0.07), and slightly increased in the FK treated group (0.27 +/- 0.07, on postoperative day 7; 0.24 +/- 0.07 at late acute rejection) compared with the syngeneic group (0.18 +/- 0.02). CONCLUSION: The activation of CD4+ and CD8+ T cells was suppressed effectively by early potent immunosuppressive treatment resulting in prolonged survival of intestinal allograft. At late acute rejection, the CD4+ and CD8+ T cells remained at low-level activation status, in contrast to the surge of CD4+ and CD8+ activation during early acute rejection. This sugg
AU - Guo,WH
AU - Tian,L
AU - Chan,KL
AU - Dallman,M
AU - Tam,PK
DO - 10.1053/jpsu.2001.20715
EP - 356
PY - 2001///
SN - 0022-3468
SP - 352
TI - Role of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft.
T2 - J Pediatr Surg
UR - http://dx.doi.org/10.1053/jpsu.2001.20715
UR - https://www.ncbi.nlm.nih.gov/pubmed/11172433
VL - 36
ER -