In this section
@article{Fabrini:2024:10.1038/s41565-024-01726-x,
author = {Fabrini, G and Farag, N and Nuccio, SP and Li, S and Stewart, JM and Tang, AA and McCoy, R and Owens, RM and Rothemund, PWK and Franco, E and Di, Antonio M and Di, Michele L},
doi = {10.1038/s41565-024-01726-x},
journal = {Nat Nanotechnol},
title = {Co-transcriptional production of programmable RNA condensates and synthetic organelles.},
url = {http://dx.doi.org/10.1038/s41565-024-01726-x},
year = {2024}
}
TY - JOUR
AB - Condensation of RNA and proteins is central to cellular functions, and the ability to program it would be valuable in synthetic biology and synthetic cell science. Here we introduce a modular platform for engineering synthetic RNA condensates from tailor-made, branched RNA nanostructures that fold and assemble co-transcriptionally. Up to three orthogonal condensates can form simultaneously and selectively accumulate fluorophores through embedded fluorescent light-up aptamers. The RNA condensates can be expressed within synthetic cells to produce membrane-less organelles with a controlled number and relative size, and showing the ability to capture proteins using selective protein-binding aptamers. The affinity between otherwise orthogonal nanostructures can be modulated by introducing dedicated linker constructs, enabling the production of bi-phasic RNA condensates with a prescribed degree of interphase mixing and diverse morphologies. The in situ expression of programmable RNA condensates could underpin the spatial organization of functionalities in both biological and synthetic cells.
AU - Fabrini,G
AU - Farag,N
AU - Nuccio,SP
AU - Li,S
AU - Stewart,JM
AU - Tang,AA
AU - McCoy,R
AU - Owens,RM
AU - Rothemund,PWK
AU - Franco,E
AU - Di,Antonio M
AU - Di,Michele L
DO - 10.1038/s41565-024-01726-x
PY - 2024///
TI - Co-transcriptional production of programmable RNA condensates and synthetic organelles.
T2 - Nat Nanotechnol
UR - http://dx.doi.org/10.1038/s41565-024-01726-x
UR - https://www.ncbi.nlm.nih.gov/pubmed/39080489
ER -