In this section
@article{Peng:2024:10.1038/s41564-023-01596-4,
author = {Peng, H and Darlington, APS and South, EJ and Chen, H-H and Jiang, W and Ledesma-Amaro, R},
doi = {10.1038/s41564-023-01596-4},
journal = {Nature Reviews Microbiology},
pages = {848--863},
title = {A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities},
url = {http://dx.doi.org/10.1038/s41564-023-01596-4},
volume = {9},
year = {2024}
}
TY - JOUR
AB - Engineered microbial consortia often have enhanced system performance and robustness compared with single-strain biomanufacturing production platforms. However, few tools are available for generating co-cultures of the model and key industrial host Saccharomyces cerevisiae. Here we engineer auxotrophic and overexpression yeast strains that can be used to create co-cultures through exchange of essential metabolites. Using these strains as modules, we engineered two- and three-member consortia using different cross-feeding architectures. Through a combination of ensemble modelling and experimentation, we explored how cellular (for example, metabolite production strength) and environmental (for example, initial population ratio, population density and extracellular supplementation) factors govern population dynamics in these systems. We tested the use of the toolkit in a division of labour biomanufacturing case study and show that it enables enhanced and tuneable antioxidant resveratrol production. We expect this toolkit to become a useful resource for a variety of applications in synthetic ecology and biomanufacturing.
AU - Peng,H
AU - Darlington,APS
AU - South,EJ
AU - Chen,H-H
AU - Jiang,W
AU - Ledesma-Amaro,R
DO - 10.1038/s41564-023-01596-4
EP - 863
PY - 2024///
SN - 1740-1526
SP - 848
TI - A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities
T2 - Nature Reviews Microbiology
UR - http://dx.doi.org/10.1038/s41564-023-01596-4
UR - https://www.ncbi.nlm.nih.gov/pubmed/38326570
UR - https://www.nature.com/articles/s41564-023-01596-4
UR - http://hdl.handle.net/10044/1/109185
VL - 9
ER -
For any enquiries about the Fungal Science Network at Imperial, please contact: