In this section
@article{Yu:2024:10.1038/s41467-024-52411-5,
author = {Yu, W and Jin, K and Wang, D and Wang, N and Li, Y and Liu, Y and Li, J and Du, G and Lv, X and Chen, J and Ledesma-Amaro, R and Liu, L},
doi = {10.1038/s41467-024-52411-5},
journal = {Nature Communications},
title = {De novo engineering of programmable and multi-functional biomolecular condensates for controlled biosynthesis},
url = {http://dx.doi.org/10.1038/s41467-024-52411-5},
volume = {15},
year = {2024}
}
TY - JOUR
AB - There is a growing interest in the creation of engineered condensates formed via liquid-liquid phase separation (LLPS) to exert precise cellular control in prokaryotes. However, de novo design of cellular condensates to control metabolic flux or protein translation remains a challenge. Here, we present a synthetic condensate platform, generated through the incorporation of artificial, disordered proteins to realize specific functions in Bacillus subtilis. To achieve this, the "stacking blocks" strategy is developed to rationally design a series of LLPS-promoting proteins for programming condensates. Through the targeted recruitment of biomolecules, our investigation demonstrates that cellular condensates effectively sequester biosynthetic pathways. We successfully harness this capability to enhance the biosynthesis of 2'-fucosyllactose by 123.3%. Furthermore, we find that condensates can enhance the translation specificity of tailored enzyme fourfold, and can increase N-acetylmannosamine titer by 75.0%. Collectively, these results lay the foundation for the design of engineered condensates endowed with multifunctional capacities.
AU - Yu,W
AU - Jin,K
AU - Wang,D
AU - Wang,N
AU - Li,Y
AU - Liu,Y
AU - Li,J
AU - Du,G
AU - Lv,X
AU - Chen,J
AU - Ledesma-Amaro,R
AU - Liu,L
DO - 10.1038/s41467-024-52411-5
PY - 2024///
SN - 2041-1723
TI - De novo engineering of programmable and multi-functional biomolecular condensates for controlled biosynthesis
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-024-52411-5
UR - https://www.ncbi.nlm.nih.gov/pubmed/39284811
UR - https://www.nature.com/articles/s41467-024-52411-5
UR - http://hdl.handle.net/10044/1/114860
VL - 15
ER -
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