In this section
@article{Park:2024:10.1038/s41467-024-53117-4,
author = {Park, Y-K and Peng, H and Hapeta, P and Sellés, Vidal L and Ledesma-Amaro, R},
doi = {10.1038/s41467-024-53117-4},
journal = {Nature Communications},
title = {Engineered cross-feeding creates inter- and intra-species synthetic yeast communities with enhanced bioproduction},
url = {http://dx.doi.org/10.1038/s41467-024-53117-4},
volume = {15},
year = {2024}
}
TY - JOUR
AB - Microorganisms can be engineered to sustainably produce a variety of products including fuels, pharmaceuticals, materials, and food. However, highly engineered strains often result in low production yield, due to undesired effects such as metabolic burden and the toxicity of intermediates. Drawing inspiration from natural ecosystems, the construction of a synthetic community with division of labor can offer advantages for bioproduction. This approach involves dividing specific tasks among community members, thereby enhancing the functionality of each member. In this study, we identify six pairs out of fifteen composed of six auxotrophs of Yarrowia lipolytica that spontaneously form robust syntrophic and synergistic communities. We characterize the stability and growth dynamics of these communities. Furthermore, we validate the existence of syntrophic interactions between two yeast species, Y. lipolytica and Saccharomyces cerevisiae, and find a strain combination, Δtrp2 and Δtrp4, forming a stable syntrophic community between two species. Subsequently, we introduce a 3-hydroxypropionic acid (3-HP) biosynthesis pathway into the syntrophic community by dividing the pathway among different strains. Our results demonstrate improved production of 3-HP in both intra- and interspecies communities compared to monocultures. Our results show the stable formation of synthetic syntrophic communities, and their potential in improving bioproduction processes.
AU - Park,Y-K
AU - Peng,H
AU - Hapeta,P
AU - Sellés,Vidal L
AU - Ledesma-Amaro,R
DO - 10.1038/s41467-024-53117-4
PY - 2024///
SN - 2041-1723
TI - Engineered cross-feeding creates inter- and intra-species synthetic yeast communities with enhanced bioproduction
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-024-53117-4
UR - https://www.nature.com/articles/s41467-024-53117-4
UR - http://hdl.handle.net/10044/1/115084
VL - 15
ER -
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