BibTex format
@article{Goosens:2021:10.1021/acssynbio.1c00358,
author = {Goosens, V and Walker, K and aragon, S and Singh, A and Senthivel, V and Dekker, L and Caro, Astorga J and Buat, M and Song, W and Lee, KY and Ellis, T},
doi = {10.1021/acssynbio.1c00358},
journal = {ACS Synthetic Biology},
pages = {3422--3434},
title = {Komagataeibacter tool kit (KTK): a modular cloning system for multigene constructs and programmed protein secretion from cellulose producing bacteria},
url = {http://dx.doi.org/10.1021/acssynbio.1c00358},
volume = {10},
year = {2021}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Bacteria proficient at producing cellulose are an attractive synthetic biology host for the emerging field of Engineered Living Materials (ELMs). Species from the Komagataeibacter genus produce high yields of pure cellulose materials in a short time with minimal resources, and pioneering work has shown that genetic engineering in these strains is possible and can be used to modify the material and its production. To accelerate synthetic biology progress in these bacteria, we introduce here the Komagataeibacter tool kit (KTK), a standardised modular cloning system based on Golden Gate DNA assembly that allows DNA parts to be combined to build complex multigene constructs expressed in bacteria from plasmids. Working in Komagataeibacter rhaeticus, we describe basic parts for this system, including promoters, fusion tags and reporter proteins, before showcasing how the assembly system enables more complex designs. Specifically, we use KTK cloning to reformat the Escherichia coli curli amyloid fibre system for functional expression in K. rhaeticus, and go on to modify it as a system for programming protein secretion from the cellulose producing bacteria. With this toolkit, we aim to accelerate modular synthetic biology in these bacteria, and enable more rapid progress in the emerging ELMs community.
AU - Goosens,V
AU - Walker,K
AU - aragon,S
AU - Singh,A
AU - Senthivel,V
AU - Dekker,L
AU - Caro,Astorga J
AU - Buat,M
AU - Song,W
AU - Lee,KY
AU - Ellis,T
DO - 10.1021/acssynbio.1c00358
EP - 3434
PY - 2021///
SN - 2161-5063
SP - 3422
TI - Komagataeibacter tool kit (KTK): a modular cloning system for multigene constructs and programmed protein secretion from cellulose producing bacteria
T2 - ACS Synthetic Biology
UR - http://dx.doi.org/10.1021/acssynbio.1c00358
UR - https://pubs.acs.org/doi/10.1021/acssynbio.1c00358
UR - http://hdl.handle.net/10044/1/92374
VL - 10
ER -
