BibTex format
@article{Chai:2018:10.1007/s13361-018-1944-8,
author = {Chai, W and Zhang, Y and Mauri, L and Ciampa, MG and Mulloy, B and Sonnino, S and Feizi, T},
doi = {10.1007/s13361-018-1944-8},
journal = {JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY},
pages = {1308--1318},
title = {Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides},
url = {http://dx.doi.org/10.1007/s13361-018-1944-8},
volume = {29},
year = {2018}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Gangliosides, as plasma membrane-associated sialylated glycolipids, are antigenic structures and they serve as ligands for adhesion proteins of pathogens, for toxins of bacteria, and for endogenous proteins of the host. The detectability by carbohydrate-binding proteins of glycan antigens and ligands on glycolipids can be influenced by the differing lipid moieties. To investigate glycan sequences of gangliosides as recognition structures, we have underway a program of work to develop a “gangliome” microarray consisting of isolated natural gangliosides and neoglycolipids (NGLs) derived from glycans released from them, and each linked to the same lipid molecule for arraying and comparative microarray binding analyses. Here, in the first phase of our studies, we describe a strategy for high-sensitivity assignment of the tetrasaccharide backbones and application to identification of eight of monosialylated glycans released from bovine brain gangliosides. This approach is based on negative-ion electrospray mass spectrometry with collision-induced dissociation (ESI-CID-MS/MS) of the desialylated glycans. Using this strategy, we have the data on backbone regions of four minor components among the monosialo-ganglioside-derived glycans; these are of the ganglio-, lacto-, and neolacto-series.
AU - Chai,W
AU - Zhang,Y
AU - Mauri,L
AU - Ciampa,MG
AU - Mulloy,B
AU - Sonnino,S
AU - Feizi,T
DO - 10.1007/s13361-018-1944-8
EP - 1318
PY - 2018///
SN - 1044-0305
SP - 1308
TI - Assignment by Negative-Ion Electrospray Tandem Mass Spectrometry of the Tetrasaccharide Backbones of Monosialylated Glycans Released from Bovine Brain Gangliosides
T2 - JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
UR - http://dx.doi.org/10.1007/s13361-018-1944-8
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000435538300023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/62757
VL - 29
ER -
