BibTex format
@article{Cecilio:2015:10.1016/j.ijbiomac.2015.09.062,
author = {Cecilio, NT and Carvalho, FC and Liu, Y and Moncrieffe, M and de, Almeida Buranello PA and Zorzetto-Fernandes, AL and Dalle, Luche D and Hanna, ES and Soares, SG and Feizi, T and Gay, NJ and Goldman, MHS and Roque-Barreira, MC},
doi = {10.1016/j.ijbiomac.2015.09.062},
journal = {International Journal of Biological Macromolecules},
pages = {22--30},
title = {Yeast expressed ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM},
url = {http://dx.doi.org/10.1016/j.ijbiomac.2015.09.062},
volume = {82},
year = {2015}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Recent advances in glycobiology have revealed the essential role of lectins in deciphering the glycocodes at the cell surface to generate important biological signaling responses. ArtinM, a d-mannose-binding lectin isolated from the seeds of jackfruit (Artocarpus heterophyllus), is composed of 16 kDa subunits that are associated to form a homotetramer. Native ArtinM (n-ArtinM) exerts immunomodulatory and regenerative effects, but the potential pharmaceutical applicability of the lectin is highly limited by the fact that its production is expensive, laborious, and impossible to be scaled up. This led us to characterize a recombinant form of the lectin obtained by expression in Saccharomyces cerevisiae (y-ArtinM). In the present study, we demonstrated that y-ArtinM is similar to n-ArtinM in subunit arrangement, oligomerization and carbohydrate binding specificity. We showed that y-ArtinM can exert n-ArtinM biological activities such as erythrocyte agglutination, stimulation of neutrophil migration and degranulation, mast cell degranulation, and induction of interleukin-12 and interleukin-10 production by macrophages. In summary, the expression of ArtinM in yeast resulted in successful production of an active, recombinant form of ArtinM that is potentially useful for pharmaceutical application.
AU - Cecilio,NT
AU - Carvalho,FC
AU - Liu,Y
AU - Moncrieffe,M
AU - de,Almeida Buranello PA
AU - Zorzetto-Fernandes,AL
AU - Dalle,Luche D
AU - Hanna,ES
AU - Soares,SG
AU - Feizi,T
AU - Gay,NJ
AU - Goldman,MHS
AU - Roque-Barreira,MC
DO - 10.1016/j.ijbiomac.2015.09.062
EP - 30
PY - 2015///
SN - 0141-8130
SP - 22
TI - Yeast expressed ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM
T2 - International Journal of Biological Macromolecules
UR - http://dx.doi.org/10.1016/j.ijbiomac.2015.09.062
UR - http://hdl.handle.net/10044/1/32572
VL - 82
ER -
