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@article{Gao:2015:10.1021/acs.analchem.5b03471,
author = {Gao, C and Zhang, Y and Liu, Y and Ten, F and Chai, W},
doi = {10.1021/acs.analchem.5b03471},
journal = {Analytical Chemistry},
pages = {11871--11878},
title = {Negative-Jon Electrospray Tandem Mass Spectrometry and Microarray Analyses of Developmentally Regulated Antigens Based on Type 1 and Type 2 Backbone Sequences},
url = {http://dx.doi.org/10.1021/acs.analchem.5b03471},
volume = {87},
year = {2015}
}

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TY  - JOUR
AB  - Type 1 (Galβ1-3GlcNAc) and type 2 (Galβ1-4GlcNAc) sequences are constituents of the backbones of alarge family of glycans of glycoproteins and glycolipids whosebranching and peripheral substitutions are developmentallyregulated. It is highly desirable to have microsequencingmethods that can be used to precisely identify and monitorthese oligosaccharide sequences with high sensitivity. Negative-ionelectrospray tandem mass spectrometry withcollision-induced dissociation has been used for characterizationof branching points, peripheral substitutions, andpartial assignment of linkages in reducing oligosaccharides. Wenow extend this method to characterizing entire sequences oflinear type 1 and type 2 chain-based glycans, focusing on thetype 1 and type 2 units in the internal regions including the linkages connecting type 1 and type 2 disaccharide units. We applythe principles to sequence analysis of closely related isomeric oligosaccharides and demonstrate by microarray analyses distinctbinding activities of antibodies and a lectin toward various combinations of type 1 and 2 units joined by 1,3- and 1,6-linkages.These sequence-specific carbohydrate-binding proteins are in turn valuable tools for detecting and distinguishing the type 1 andtype 2-based developmentally regulated glycan sequences.
AU  - Gao,C
AU  - Zhang,Y
AU  - Liu,Y
AU  - Ten,F
AU  - Chai,W
DO  - 10.1021/acs.analchem.5b03471
EP  - 11878
PY  - 2015///
SN  - 1520-6882
SP  - 11871
TI  - Negative-Jon Electrospray Tandem Mass Spectrometry and Microarray Analyses of Developmentally Regulated Antigens Based on Type 1 and Type 2 Backbone Sequences
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.5b03471
VL  - 87
ER  -