BibTex format
@article{Bartels:2016:10.1371/journal.pone.0166119,
author = {Bartels, MF and Winterhalter, PR and Yu, J and Liu, Y and Lommel, M and Möhrlen, F and Hu, H and Feizi, T and Westerlind, U and Ruppert, T and Strahl, S},
doi = {10.1371/journal.pone.0166119},
journal = {PLOS One},
title = {Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates},
url = {http://dx.doi.org/10.1371/journal.pone.0166119},
volume = {11},
year = {2016}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Protein O-mannosylation is a post-translational modification essential for correct development of mammals. In humans, deficient O-mannosylation results in severe congenital muscular dystrophies often associated with impaired brain and eye development. Although various O-mannosylated proteins have been identified in the recent years, the distribution of O-mannosyl glycans in the mammalian brain and target proteins are still not well defined. In the present study, rabbit monoclonal antibodies directed against the O-mannosylated peptide YAT(α1-Man)AV were generated. Detailed characterization of clone RKU-1-3-5 revealed that this monoclonal antibody recognizes O-linked mannose also in different peptide and protein contexts. Using this tool, we observed that mono-O-mannosyl glycans occur ubiquitously throughout the murine brain but are especially enriched at inhibitory GABAergic neurons and at the perineural nets. Using a mass spectrometry-based approach, we further identified glycoproteins from the murine brain that bear single O-mannose residues. Among the candidates identified are members of the cadherin and plexin superfamilies and the perineural net protein neurocan. In addition, we identified neurexin 3, a cell adhesion protein involved in synaptic plasticity, and inter-alpha-trypsin inhibitor 5, a protease inhibitor important in stabilizing the extracellular matrix, as new O-mannosylated glycoproteins.
AU - Bartels,MF
AU - Winterhalter,PR
AU - Yu,J
AU - Liu,Y
AU - Lommel,M
AU - Möhrlen,F
AU - Hu,H
AU - Feizi,T
AU - Westerlind,U
AU - Ruppert,T
AU - Strahl,S
DO - 10.1371/journal.pone.0166119
PY - 2016///
SN - 1932-6203
TI - Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates
T2 - PLOS One
UR - http://dx.doi.org/10.1371/journal.pone.0166119
UR - http://hdl.handle.net/10044/1/42406
VL - 11
ER -
