@article{Chayen:2016:10.1038/srep20053, author = {Chayen, N and shaffer and govada and Khurshid and Kassen and Leese, H S and Hu, S and Menzel and Chain, B and Saridakis}, doi = {10.1038/srep20053}, journal = {Scientific Reports}, title = {Exploring Carbon Nanomaterial Diversity for Nucleation of Protein Crystals}, url = {http://dx.doi.org/10.1038/srep20053}, volume = {6}, year = {2016} }
TY - JOUR AB - Controlling crystal nucleation is a crucial step in obtaining high quality protein crystals for structure determination by X-ray crystallography. Carbon nanomaterials (CNMs) including carbon nanotubes, graphene oxide, and carbon black provide a range of surface topographies, porosities and length scales; functionalisation with two different approaches, gas phase radical grafting and liquid phase reductive grafting, provide routes to a range of oligomer functionalised products. These grafted materials, combined with a range of controls, were used in a large-scale assessment of the effectiveness for protein crystal nucleation of 20 different carbon nanomaterials on five proteins. This study has allowed a direct comparison of the key characteristics of carbon-based nucleants: appropriate surface chemistry, porosity and/or roughness are required. The most effective solid system tested in this study, carbon black nanoparticles functionalised with poly(ethylene glycol) methyl ether of mean molecular weight 5000, provides a novel highly effective nucleant, that was able to induce crystal nucleation of four out of the five proteins tested at metastable conditions. AU - Chayen,N AU - shaffer AU - govada AU - Khurshid AU - Kassen AU - Leese,H S AU - Hu,S AU - Menzel AU - Chain,B AU - Saridakis DO - 10.1038/srep20053 PY - 2016/// SN - 2045-2322 TI - Exploring Carbon Nanomaterial Diversity for Nucleation of Protein Crystals T2 - Scientific Reports UR - http://dx.doi.org/10.1038/srep20053 UR - http://hdl.handle.net/10044/1/28245 VL - 6 ER -
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