@article{Pennisi:2024:10.1021/acs.analchem.4c00319,
author = {Pennisi, I and Cavuto, ML and Miglietta, L and Malpartida-Cardenas, K and Stringer, OW and Mantikas, K-T and Reid, R and Frise, R and Moser, N and Randell, P and Davies, F and Bolt, F and Barclay, W and Holmes, A and Georgiou, P and Rodriguez-Manzano, J},
doi = {10.1021/acs.analchem.4c00319},
journal = {Analytical Chemistry},
pages = {11181--11188},
title = {Rapid, portable, and electricity-free sample extraction method for enhanced molecular diagnostics in resource-limited settings},
url = {http://dx.doi.org/10.1021/acs.analchem.4c00319},
volume = {96},
year = {2024}
}

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TY  - JOUR
AB  - The COVID-19 pandemic has highlighted the need for rapid and reliable diagnostics that are accessible in resource-limited settings. To address this pressing issue, we have developed a rapid, portable, and electricity-free method for extracting nucleic acids from respiratory swabs (i.e. nasal, nasopharyngeal and buccal swabs), successfully demonstrating its effectiveness for the detection of SARS-CoV-2 in residual clinical specimens. Unlike traditional approaches, our solution eliminates the need for micropipettes or electrical equipment, making it user-friendly and requiring little to no training. Our method builds upon the principles of magnetic bead extraction and revolves around a low-cost plastic magnetic lid, called SmartLid, in combination with a simple disposable kit containing all required reagents conveniently prealiquoted. Here, we clinically validated the SmartLid sample preparation method in comparison to the gold standard QIAamp Viral RNA Mini Kit from QIAGEN, using 406 clinical isolates, including 161 SARS-CoV-2 positives, using the SARS-CoV-2 RT-qPCR assays developed by the US Centers for Disease Control and Prevention (CDC). The SmartLid method showed an overall sensitivity of 95.03% (95% CI: 90.44-97.83%) and a specificity of 99.59% (95% CI: 97.76-99.99%), with a positive agreement of 97.79% (95% CI: 95.84-98.98%) when compared to QIAGEN's column-based extraction method. There are clear benefits to using the SmartLid sample preparation kit: it enables swift extraction of viral nucleic acids, taking less than 5 min, without sacrificing significant accuracy when compared to more expensive and time-consuming alternatives currently available on the market. Moreover, its simplicity makes it particularly well-suited for the point-of-care where rapid results and portability are crucial. By providing an efficient and accessible means of nucleic acid extraction, our approach aims to introduce a step-change in diagnostic capabilities for resource-limited sett
AU  - Pennisi,I
AU  - Cavuto,ML
AU  - Miglietta,L
AU  - Malpartida-Cardenas,K
AU  - Stringer,OW
AU  - Mantikas,K-T
AU  - Reid,R
AU  - Frise,R
AU  - Moser,N
AU  - Randell,P
AU  - Davies,F
AU  - Bolt,F
AU  - Barclay,W
AU  - Holmes,A
AU  - Georgiou,P
AU  - Rodriguez-Manzano,J
DO  - 10.1021/acs.analchem.4c00319
EP  - 11188
PY  - 2024///
SN  - 0003-2700
SP  - 11181
TI  - Rapid, portable, and electricity-free sample extraction method for enhanced molecular diagnostics in resource-limited settings
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.4c00319
UR  - https://www.ncbi.nlm.nih.gov/pubmed/38967089
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.4c00319
UR  - http://hdl.handle.net/10044/1/113336
VL  - 96
ER  - 

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