@article{King:2015:10.1371/journal.pone.0141557,
author = {King, DFL and McKay, PF and Mann, JFS and Jones, CB and Shattock, RJ},
doi = {10.1371/journal.pone.0141557},
journal = {PLOS One},
title = {Plasmid DNA Vaccine Co-Immunisation Modulates Cellular and Humoral Immune Responses Induced by Intranasal Inoculation in Mice},
url = {http://dx.doi.org/10.1371/journal.pone.0141557},
volume = {10},
year = {2015}
}

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TY  - JOUR
AB  - BackgroundAn effective HIV vaccine will likely require induction of both mucosal and systemic cellularand humoral immune responses. We investigated whether intramuscular (IM) delivery ofelectroporated plasmid DNA vaccine and simultaneous protein vaccinations by intranasal(IN) and IM routes could be combined to induce mucosal and systemic cellular and humoralimmune responses to a model HIV-1 CN54 gp140 antigen in mice.ResultsCo-immunisation of DNA with intranasal protein successfully elicited both serum and vaginalIgG and IgA responses, whereas DNA and IM protein co-delivery did not induce systemicor mucosal IgA responses. Cellular IFNγ responses were preserved in coimmunisationprotocols compared to protein-only vaccination groups. The addition of DNAto IN protein vaccination reduced the strong Th2 bias observed with IN protein vaccinationalone. Luminex analysis also revealed that co-immunisation with DNA and IN proteininduced expression of cytokines that promote B-cell function, generation of TFH cells andCCR5 ligands that can reduce HIV infectivity.SignificanceThese data suggest that while IN inoculation alone elicits both cellular and humoralresponses, co-administration with homologous DNA vaccination can tailor these towards amore balanced Th1/Th2 phenotype modulating the cellular cytokine profile while elicitinghigh-levels of antigen-specific antibody. This work provides insights on how to generate differentialimmune responses within the same vaccination visit, and supports co-immunisationwith DNA and protein by a mucosal route as a potential delivery strategy for HIVvaccines.
AU  - King,DFL
AU  - McKay,PF
AU  - Mann,JFS
AU  - Jones,CB
AU  - Shattock,RJ
DO  - 10.1371/journal.pone.0141557
PY  - 2015///
SN  - 1932-6203
TI  - Plasmid DNA Vaccine Co-Immunisation Modulates Cellular and Humoral Immune Responses Induced by Intranasal Inoculation in Mice
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0141557
UR  - http://hdl.handle.net/10044/1/28662
VL  - 10
ER  - 

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