@article{Lambert:2016:10.3389/fimmu.2016.00321,
author = {Lambert, L and Kinnear, E and Mcdonald, JU and Grodeland, G and Bogen, B and Stubsrud, E and Lindberg, MM and Brunsvik, Frediksen A and Tregoning, JS},
doi = {10.3389/fimmu.2016.00321},
journal = {Frontiers in Immunology},
title = {DNA Vaccines Encoding Antigen Targeted to MHC Class II Induce Influenza-Specific CD8+ T Cell Responses, Enabling Faster Resolution of Influenza Disease},
url = {http://dx.doi.org/10.3389/fimmu.2016.00321},
volume = {7},
year = {2016}
}

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TY  - JOUR
AB  - Current influenza vaccines are effective but imperfect, failing to cover against emerging strains of virus and requiring seasonaladministration to protect against new strains. A key step to improving influenza vaccines is to improve our understanding ofvaccine induced protection. Whilst it is clear that antibodies play a protective role, vaccine induced CD8+ T cells can improveprotection. To further explore the role of CD8+ T cells we used a DNA vaccine that encodes antigen dimerised to an immune celltargeting module. Immunising CB6F1 mice with the DNA vaccine in a heterologous prime boost regime with the seasonal proteinvaccine improved the resolution of influenza disease compared to protein alone. This improved disease resolution was dependenton CD8+ T cells. However, DNA vaccine regimes that induced CD8+ T cells alone were not protective and did not boost theprotection provided by protein. The MHC targeting module used was an anti-I-Ed single chain antibody specific to the BALB/c strainof mice. To test the role of MHC targeting we compared the response between BALB/c, C57BL/6 mice and an F1 cross of the twostrains (CB6F1). BALB/c mice were protected, C57BL/6 were not and the F1 had an intermediate phenotype; showing that thetargeting of antigen is important in the response. Based on these findings, and in agreement with other studies using differentvaccines, we conclude that in addition to antibody, inducing a protective CD8 response is important in future influenza vaccines.
AU  - Lambert,L
AU  - Kinnear,E
AU  - Mcdonald,JU
AU  - Grodeland,G
AU  - Bogen,B
AU  - Stubsrud,E
AU  - Lindberg,MM
AU  - Brunsvik,Frediksen A
AU  - Tregoning,JS
DO  - 10.3389/fimmu.2016.00321
PY  - 2016///
SN  - 1664-3224
TI  - DNA Vaccines Encoding Antigen Targeted to MHC Class II Induce Influenza-Specific CD8+ T Cell Responses, Enabling Faster Resolution of Influenza Disease
T2  - Frontiers in Immunology
UR  - http://dx.doi.org/10.3389/fimmu.2016.00321
UR  - http://hdl.handle.net/10044/1/38854
VL  - 7
ER  - 

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