BibTex format
@article{Wood:2019:10.1016/j.celrep.2019.08.094,
author = {Wood, TE and Howard, SA and Forster, A and Nolan, LM and Manoli, E and Bullen, NP and Yau, HCL and Hachani, A and Hayward, RD and Whitney, JC and Vollmer, W and Freemont, PS and Filloux, A},
doi = {10.1016/j.celrep.2019.08.094},
journal = {Cell Reports},
pages = {187--201.e7},
title = {The Pseudomonas aeruginosa T6SS delivers a periplasmic toxin that disrupts bacterial cell morphology},
url = {http://dx.doi.org/10.1016/j.celrep.2019.08.094},
volume = {29},
year = {2019}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - The type VI secretion system (T6SS) is crucialin interbacterial competition and is avirulence determinant ofmany Gram-negative bacteria. Several T6SS effectorsarecovalently fused to secreted T6SS structural components such asthe VgrG spike for delivery into target cells.In Pseudomonas aeruginosa, theVgrG2b effector waspreviously proposedto mediatebacterial internalisation into eukaryotic cells. In this work, wefind that the VgrG2b C-terminal domain(VgrG2bC-ter) elicits toxicity in the bacterial periplasm, counteracted by a cognate immunity protein.We resolve thestructure of VgrG2bC-ter and confirm it is a member ofthezinc-metallopeptidasefamily of enzymes. We show that this effector causesmembrane blebbing atmidcell, whichsuggests a distincttype of T6SS-mediated growthinhibition through interference with cell division, mimicking the impact of β-lactam antibiotics. Ourstudyintroduces a further effector family to the T6SS arsenaland demonstrates that VgrG2b can target both prokaryotic and eukaryotic cells.
AU - Wood,TE
AU - Howard,SA
AU - Forster,A
AU - Nolan,LM
AU - Manoli,E
AU - Bullen,NP
AU - Yau,HCL
AU - Hachani,A
AU - Hayward,RD
AU - Whitney,JC
AU - Vollmer,W
AU - Freemont,PS
AU - Filloux,A
DO - 10.1016/j.celrep.2019.08.094
EP - 201
PY - 2019///
SN - 2211-1247
SP - 187
TI - The Pseudomonas aeruginosa T6SS delivers a periplasmic toxin that disrupts bacterial cell morphology
T2 - Cell Reports
UR - http://dx.doi.org/10.1016/j.celrep.2019.08.094
UR - https://www.sciencedirect.com/science/article/pii/S2211124719311568?via%3Dihub
UR - http://hdl.handle.net/10044/1/73329
VL - 29
ER -