In this section
@article{Aylett:2021:10.1016/j.jsb.2020.107677,
author = {Aylett, C},
doi = {10.1016/j.jsb.2020.107677},
journal = {Journal of Structural Biology},
title = {Direct transfer of electron microscopy samples to wetted carbon and graphene films via a support floatation block},
url = {http://dx.doi.org/10.1016/j.jsb.2020.107677},
volume = {213},
year = {2021}
}
TY - JOUR
AB - Support films are commonly used during cryo-EM specimen preparation to both immobilise the sample and minimise the exposure of particles at the air-water interface. Here we report preparation protocols for carbon and graphene supported single particle electron microscopy samples using a novel 3D-printed sample transfer block to facilitate the direct, wetted, movement of both carbon and graphene supports from the substrate on which they were generated to small volumes (10 μL) of sample. These approaches are simple and inexpensive to implement, minimise hydrophobic contamination of the support films, and are widely applicable to single particle studies. Our approach also allows the direct exchange of the sample buffer on the support film in cases in which it is unsuitable for vitrification, e.g. for samples from centrifugal density gradients that help to preserve sample integrity.
AU - Aylett,C
DO - 10.1016/j.jsb.2020.107677
PY - 2021///
SN - 1047-8477
TI - Direct transfer of electron microscopy samples to wetted carbon and graphene films via a support floatation block
T2 - Journal of Structural Biology
UR - http://dx.doi.org/10.1016/j.jsb.2020.107677
UR - https://www.sciencedirect.com/science/article/pii/S1047847720302501
UR - http://hdl.handle.net/10044/1/86033
VL - 213
ER -
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