The BD FACSAria III in the Flowers Building is offered for CL2 work, including bacteria and yeast. Please refer to the Biosafety page (link at the bottom of this page) for more information on the documentation needed to use this instrument.

The Flowers FACSAria III is a 14 colour high speed cell sorter with four lasers (Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 640nm) enclosed in a hood for Class II work, a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled cuvette as opposed to the jet-in-air system, and this combined with trigon and octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength.

A choice of nozzles lets users sort a wide range of particle sizes, from bacteria to large white blood cells. It is equipped with a versatile sort collection chamber that allows users to sort into tubes, slides and plates. Cooling and heating for sort collection are available.

Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 70,000 events/second. We recommend a minimum sample volume of 500 μL.

For more information, please read the BD FACSAria III Cell Sorter Technical Specifications.

Download a complete configuration of the Aria III Flowers Lasers and Filters, including fluorochromes and bandpass filters. You can also find it below.

Location

Flowers Building, 3^rd Floor, Lab 3.3.

The Aria III is operated by the facility staff only, with 9am-to-6pm access.