BD FACSAria Fusion

The BD FACSAria Fusion is offered for CL2 work with cultured cells and patient samples only. For work with bacteria and yeast please consult our other cell sorters. Please refer to the Biosafety page (link at the bottom of this page) for more information on the documentation needed to use this instrument.

The FACSAria Fusion is a 14-colour high speed cell sorter with three lasers (Violet 405nm, Blue 488nm and Red 640nm), an integrated biosafety cabinet, a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled cuvette as opposed to the jet-in-air system, and this combined with octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength. To further enhance biosafety protection, the BD FACSAria Fusion has an integrated biosafety cabinet a built-in Aerosol Management System (AMS) that evacuates aerosols.

A choice of nozzles lets users sort a wide range of cell sizes. It is equipped with a versatile sort collection chamber that allows users to sort into tubes, slides and plates. Cooling and heating for sort collection are available.

Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 70,000 events/second. We recommend a minimum sample volume of 500 μL.

For more information, please read the BD FACSAria Fusion technical specifications‌.

Download a complete configuration of the Fusion Lasers and Filters‌, including fluorochromes and bandpass filters. You can also find it below.

Location

The Royal Brompton hospital, Guy Scadding building. Please book via the St Mary’s Flow Facility PPMS.

The Fusion is available to any trained user 24 hours a day (subject to building access and regulations).