@article{Beltrán:2024:10.1128/mbio.02857-23,
author = {Beltrán, L and Torsilieri, H and Patkowski, JB and Yang, JE and Casanova, J and Costa, TRD and Wright, ER and Egelman, EH},
doi = {10.1128/mbio.02857-23},
journal = {mBio},
title = {The mating pilus of E. coli pED208 acts as a conduit for ssDNA during horizontal gene transfer},
url = {http://dx.doi.org/10.1128/mbio.02857-23},
volume = {15},
year = {2024}
}

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TY  - JOUR
AB  - Bacteria are constantly exchanging DNA, which constitutes horizontal gene transfer. While some of these occurs by a non-specific process called natural transformation, some occurs by a specific mating between a donor and a recipient cell. In specific conjugation, the mating pilus is extended from the donor cell to make contact with the recipient cell, but whether DNA is actually transferred through this pilus or by another mechanism involving the type IV secretion system complex without the pilus has been an open question. Using Escherichia coli, we show that DNA can be transferred through this pilus between a donor and a recipient cell that has not established a tight mating junction, providing a new picture for the role of this pilus.
AU  - Beltrán,L
AU  - Torsilieri,H
AU  - Patkowski,JB
AU  - Yang,JE
AU  - Casanova,J
AU  - Costa,TRD
AU  - Wright,ER
AU  - Egelman,EH
DO  - 10.1128/mbio.02857-23
PY  - 2024///
SN  - 2150-7511
TI  - The mating pilus of E. coli pED208 acts as a conduit for ssDNA during horizontal gene transfer
T2  - mBio
UR  - http://dx.doi.org/10.1128/mbio.02857-23
UR  - https://www.ncbi.nlm.nih.gov/pubmed/38051116
UR  - https://journals.asm.org/doi/10.1128/mbio.02857-23
UR  - http://hdl.handle.net/10044/1/108853
VL  - 15
ER  - 

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