In this section
@article{Grob:2024:10.1038/s41467-023-44396-4,
author = {Grob, A and Enrico, Bena C and Di, Blasi R and Pessina, D and Sood, M and Yunyue, Z and Bosia, C and Isalan, M and Ceroni, F},
doi = {10.1038/s41467-023-44396-4},
journal = {Nature Communications},
title = {Mammalian cell growth characterisation by a non-invasive plate reader assay},
url = {http://dx.doi.org/10.1038/s41467-023-44396-4},
volume = {15},
year = {2024}
}
TY - JOUR
AB - Automated and non-invasive mammalian cell analysis is currently lagging behind due to a lack of methods suitable for a variety of cell lines and applications. Here, we report the development of a high throughput non-invasive method for tracking mammalian cell growth and performance based on plate reader measurements. We show the method to be suitable for both suspension and adhesion cell lines, and we demonstrate it can be adopted when cells are grown under different environmental conditions. We establish that the method is suitable to inform on effective drug treatments to be used depending on the cell line considered, and that it can support characterisation of engineered mammalian cells over time. This work provides the scientific community with an innovative approach to mammalian cell screening, also contributing to the current efforts towards high throughput and automated mammalian cell engineering.
AU - Grob,A
AU - Enrico,Bena C
AU - Di,Blasi R
AU - Pessina,D
AU - Sood,M
AU - Yunyue,Z
AU - Bosia,C
AU - Isalan,M
AU - Ceroni,F
DO - 10.1038/s41467-023-44396-4
PY - 2024///
SN - 2041-1723
TI - Mammalian cell growth characterisation by a non-invasive plate reader assay
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-023-44396-4
UR - https://www.ncbi.nlm.nih.gov/pubmed/38167870
UR - http://hdl.handle.net/10044/1/109959
VL - 15
ER -
Interested in studying a PhD at the Department of Life Sciences? Find out more about postgraduate research opportunties.