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@article{Ahmadi:2024:10.1016/j.biosx.2023.100436,
author = {Ahmadi, Y and Umrekar, TR and Mutter, N and Beeby, M and Barii, I},
doi = {10.1016/j.biosx.2023.100436},
journal = {Biosensors and Bioelectronics: X},
title = {DNA origami-enhanced binding of aptamers to Staphylococcus aureus cells},
url = {http://dx.doi.org/10.1016/j.biosx.2023.100436},
volume = {16},
year = {2024}
}
TY - JOUR
AB - The combination of DNA origami nanostructures and aptamers provides a powerful technology for diagnostic assays. Here, we functionalized a DNA origami nanostructure with a Protein-A binding aptamer to target Staphylococcus aureus bacterial cells. Using an enzyme-linked oligonucleotide assay (ELONA), we semi-quantitatively analyzed and compared the interaction of the aptamer and aptamer-modified DNA origamis with Staphylococcus aureus bacterial isolates. The results showed that aptamer-functionalized DNA nanostructures bind with five times higher affinity (KD: 34 ± 5 nM) compared to the aptamer alone (KD: 160 ± 9 nM). Visualising the interaction of bacterial cells and nanostructures with electron cryotomography further confirmed the aptamer-mediated specific interaction of DNA nanostructures with bacterial cells.
AU - Ahmadi,Y
AU - Umrekar,TR
AU - Mutter,N
AU - Beeby,M
AU - Barii,I
DO - 10.1016/j.biosx.2023.100436
PY - 2024///
TI - DNA origami-enhanced binding of aptamers to Staphylococcus aureus cells
T2 - Biosensors and Bioelectronics: X
UR - http://dx.doi.org/10.1016/j.biosx.2023.100436
VL - 16
ER -
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