@inbook{Qi:2024:10.1007/978-1-0716-3718-0_1,
author = {Qi, L and Bennett, E and Isalan, M},
booktitle = {Mammalian synthetic systems},
doi = {10.1007/978-1-0716-3718-0_1},
editor = {Ceroni and Polizzi},
pages = {1--13},
publisher = {Springer},
title = {A directed evolution protocol for engineering minimal transcription factors, based on CIS display},
url = {http://dx.doi.org/10.1007/978-1-0716-3718-0_1},
year = {2024}
}

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TY  - CHAP
AB  - Directed evolution is an efficient strategy for obtaining desired biomolecules. Since the 1990s, the emergence of display techniques has enabled high-throughput screening of functional proteins. However, classical methods require library construction by plasmid cloning and are limited by transformation efficiencies, typically limiting library sizes to ~106–107 variants. More recently, in vitro techniques have emerged that avoid cloning, allowing library sizes of >1012 members. One of these, CIS display, is a DNA-based display technique which allows high-throughput selection of biomolecules in vitro. CIS display creates the genotype–phenotype link required for selection by a DNA replication initiator protein, RepA, that binds exclusively to the template from which it has been expressed. This method has been successfully used to evolve new protein–protein interactions but has not been used before to select DNA-binding proteins, which are major components in mammalian synthetic biology. In this chapter, we describe a directed evolution method using CIS display to efficiently select functional DNA-binding proteins from pools of nonbinding proteins. The method is illustrated by enriching the minimal transcription factor Cro from a low starting frequency (1 in 109). This protocol is also applicable to engineering other DNA-binding proteins or transcription factors from combinatorial libraries.
AU  - Qi,L
AU  - Bennett,E
AU  - Isalan,M
DO  - 10.1007/978-1-0716-3718-0_1
EP  - 13
PB  - Springer
PY  - 2024///
SN  - 978-1-0716-3718-0
SP  - 1
TI  - A directed evolution protocol for engineering minimal transcription factors, based on CIS display
T1  - Mammalian synthetic systems
UR  - http://dx.doi.org/10.1007/978-1-0716-3718-0_1
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-3718-0_1#citeas
UR  - http://hdl.handle.net/10044/1/111115
ER  - 

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