In this section
@inbook{McArthur:2024:10.1016/bs.mcb.2024.03.002,
author = {McArthur, HCW and Bajur, AT and Spillane, KM},
booktitle = {Methods in Cell Biology},
doi = {10.1016/bs.mcb.2024.03.002},
title = {Quantifying force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors},
url = {http://dx.doi.org/10.1016/bs.mcb.2024.03.002},
year = {2024}
}
TY - CHAP
AB - B cells exert pulling forces against antigen-presenting cells (APCs) to extract antigens for internalization. The application of tugging forces on B cell receptor (BCR)-antigen bonds promotes discrimination of antigen affinities and sensing of APC physical properties. Here, we describe a protocol for preparing antigen-functionalized DNA tension sensors for quantifying force-mediated antigen extraction in the B cell immune synapse. We describe how to attach the sensors to planar lipid bilayers, quantify their surface density, use them to stimulate B cell activation, and analyze the efficiency of antigen extraction in fixed cells by fluorescence microscopy and image analysis. These techniques should be broadly applicable to studies of force-mediated transfer of molecules in cell-cell contacts.
AU - McArthur,HCW
AU - Bajur,AT
AU - Spillane,KM
DO - 10.1016/bs.mcb.2024.03.002
PY - 2024///
TI - Quantifying force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors
T1 - Methods in Cell Biology
UR - http://dx.doi.org/10.1016/bs.mcb.2024.03.002
ER -
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